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Journal of Muscle Research & Cell Motility

, Volume 6, Issue 4, pp 507–512 | Cite as

Resolution of the competitive inhibitory effects of lithium and AMPPNP on the beat frequency of ATP-reactivated, demembranated, sea urchin sperm flagella

  • E. F. Pate
  • C. J. Brokaw
Papers

Summary

About half of the inhibitory effect on the beat frequency of sea urchin spermatozoa flagella by β, γ-imido-adenosine-5′-triphosphate (AMPPNP) previously reported by Okuno & Brokaw (1981) was actually the result of inhibition by lithium resulting from the use of a tetralithium salt of AMPPNP. Li+ (or LiATP3−) acts as a competitive inhibitor of beat frequency, with aKi for Li+ of about 30 mM. Under improved reactivation conditions which reduce theKm for beat frequency to about 0.1 mM, theKi for lithium-free MgAMPPNP2− is about 2.3 mM, and magnesium pyrophosphate (MgPP i ), which may be an equivalent ligand, gives aKi of 3.4 mM.

Keywords

Magnesium Lithium Triphosphate Pyrophosphate Competitive Inhibitor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall Ltd. 1985

Authors and Affiliations

  • E. F. Pate
    • 1
  • C. J. Brokaw
    • 1
  1. 1.Division of BiologyCalifornia Institute of TechnologyPasadenaUSA

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