Summary
About half of the inhibitory effect on the beat frequency of sea urchin spermatozoa flagella by β, γ-imido-adenosine-5′-triphosphate (AMPPNP) previously reported by Okuno & Brokaw (1981) was actually the result of inhibition by lithium resulting from the use of a tetralithium salt of AMPPNP. Li+ (or LiATP3−) acts as a competitive inhibitor of beat frequency, with aK i for Li+ of about 30 mM. Under improved reactivation conditions which reduce theK m for beat frequency to about 0.1 mM, theK i for lithium-free MgAMPPNP2− is about 2.3 mM, and magnesium pyrophosphate (MgPP −i ), which may be an equivalent ligand, gives aK i of 3.4 mM.
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Pate, E.F., Brokaw, C.J. Resolution of the competitive inhibitory effects of lithium and AMPPNP on the beat frequency of ATP-reactivated, demembranated, sea urchin sperm flagella. J Muscle Res Cell Motil 6, 507–512 (1985). https://doi.org/10.1007/BF00712586
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DOI: https://doi.org/10.1007/BF00712586