Journal of Muscle Research & Cell Motility

, Volume 4, Issue 5, pp 543–556 | Cite as

Generation of tension by glycerol-extracted vertebrate skeletal muscle fibres in the absence of calcium

  • H. D. Loxdale
  • R. T. Tregear
Papers

Summary

When a small bundle of glycerol-extracted fibres from either frog, tortoise or rabbit skeletal muscle was first exposed to high MgATP (5mm) in the absence of Ca2+ (<1nm) and at low ionic strength (<0.11) at 20° C, it produced a single sharp transient contraction followed by a lower maintained isometric tension. The maintained tension was investigated further in rabbit psoas fibres. Ca2+-free tension was dependent on the ionic strength in the range 0.04–0.10, on the temperature in the range 6–20° C and the free Mg2+ in the range 0–6mm. It was promoted by low ionic strength, low Mg2+ and high temperature, and was unaffected by varying the MgATP2− in the range 0.4–4mm and by adding ATP regenerating components. A separate regime of tension generation was detected at MgATP2− concentrations of less than 0.1mm, in which MgATP2− concentration was critical. The results are interpreted on the assumption that binding of Mg2+ to some component of the regulatory system is necessary to maintain its inhibitory effect in the absence of Ca2+. Ionic strength and temperature, on the other hand, may affect actomyosin directly.

Keywords

Calcium Skeletal Muscle Muscle Fibre Ionic Strength Regulatory System 

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Copyright information

© Chapman and Hall Ltd 1983

Authors and Affiliations

  • H. D. Loxdale
    • 1
  • R. T. Tregear
    • 2
  1. 1.Zoology DepartmentUniversity of OxfordOxfordUK
  2. 2.ARC Institute of Animal PhysiologyCambridgeUK

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