Summary
This paper outlines the results of the first homodyning low angle scattering experiment known to have been reported for motile particles. Low angle scattering QELS studies were carried out on a wild strain ofChlamydomonas reinhardtii at scattering angles of 3.3°, 2.6° and 1.7°. The resultant experimental autocorrelation functions and scaling curves were compared with a theoretical model that had been successful previously at scattering angles greater than 15°.
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CHEN, S.-H. & HALLETT, F. R. (1982) Determination of motile behaviour of prokaryotic and eukaryotic cells by quasi-elastic light scattering.Quart. Rev. Biophys. 15, 131–222.
CRAIG, T. & HALLETT, F. R. (1982) Half-width scaling of electric field autocorrelation functions of light scattered from bull spermatozoa.Biophys. J. 38, 71–8.
CRAIG, T., HALLETT, F. R. & CHEN, S.-H. (1982) Scaling properties of light scattering spectra for particles with helical trajectories.Biophys. J. 38, 63–70.
CRAIG, T., HALLETT, F. R. & NICKEL, B. (1979) Quasi-elastic light scattering spectra of swimming spermatozoa. Rotational and translational effects.Biophys. J. 28, 457–72.
HOLZ, M. & CHEN, S.-H. (1978) Rotational-translational models for interpretation of quasi-elastic light scattering spectra of motile bacteria.Appl. Opt. 17, 3197–204.
NOSSAL, R. (1971) Spectral analysis of laser light scattered from motile organisms.Biophys. J. 11, 341–54.
NOSSAL, R., CHEN, S.-H. & LAI, C.-C. (1971) Use of laser scattering for quantitative determination of bacterial motility.Opt. Comm. 4, 35–9.
RACEY, T. J. (1982) An oscillating-translational analysis of the quasi-elastic light scattering spectrum fromChlamydomonas reinhardtii PhD thesis, University of Guelph.
RACEY, T. J. & HALLETT, F. R. (1983) A quasi-elastic light scattering and cinematographical comparison of three strains of motileChlamydomonas reinhardtii: a wild type strain, a colchine resistant mutant and a backward swimming mutant.J. Musc. Res. Cell Motility 4, 333–51.
RACEY, T. J., HALLETT, F. R. & NICKEL, B. (1981) A quasi-elastic light scattering and cinematographical investigation of motileChlamydomonas reinhardtii.Biophys. J. 35, 557–71.
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Racey, T.J., Hallett, F.R. A low angle quasi-elastic light scattering investigation ofChlamydomonas reinhardtii . J Muscle Res Cell Motil 4, 321–331 (1983). https://doi.org/10.1007/BF00711999
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DOI: https://doi.org/10.1007/BF00711999