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The effect of cytochalasin and glutaraldehyde on F-actin filaments containing muscle and non-muscle tropomyosin

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Summary

F-actin filaments are disrupted by the action of cytochalasin and glutaraldehyde. Muscle tropomyosin which is able to polymerize can protect F-actin against fragmentation caused by these two agents. This protective effect does not occur with nonpolymerizable, brain or carboxypeptidase A-treated skeletal muscle tropomyosins. The protection of F-actin against the action of cytochalasin and glutaraldehyde takes place under conditions where the F-actin filaments are saturated with tropomyosin, that is, at a molar ratio of tropomyosin to actin of 1 : 7. It is suggested that nonpolymerizable tropomyosin lacks the protective ability because its binding to F-actin is considerably weaker than the polymerizable tropomyosin and does not saturate all of the binding sites on F-actin.

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  1. Ewa Próchniewicz

    Present address: Department of Biophysical Engineering, Faculty of Engineering Sciences, Osaka University, 560, Toyonaka, Osaka, Japan

Authors and Affiliations

  1. Department of Biochemistry of Nervous System and Muscle, Nencki Institute of Experimental Biology, Warsaw, Poland

    Renata Dabrowska, Ewa Próchniewicz & W. Drabikowski

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  1. Renata Dabrowska
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  2. Ewa Próchniewicz
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  3. W. Drabikowski
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Dabrowska, R., Próchniewicz, E. & Drabikowski, W. The effect of cytochalasin and glutaraldehyde on F-actin filaments containing muscle and non-muscle tropomyosin. J Muscle Res Cell Motil 4, 83–93 (1983). https://doi.org/10.1007/BF00711959

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  • DOI: https://doi.org/10.1007/BF00711959

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