Journal of Muscle Research & Cell Motility

, Volume 1, Issue 3, pp 279–303 | Cite as

Sinusoidal analysis: a high resolution method for correlating biochemical reactions with physiological processes in activated skeletal muscles of rabbit, frog and crayfish

  • Masataka Kawai
  • Philip W. Brandt
Papers

Summary

A high resolution method for determining the complex stiffness of single muscle fibres is described. In this method the length of the fibre is oscillated sinusoidally, and the resulting force amplitude and phase shift are observed and interpreted in terms of chemo-mechanical energy transduction. In activated, fast skeletal muscles of rabbit (psoas), frog (semitendinosus) and crayfish (walking leg flexor), we resolved at least three exponential rate processes. We named these (A), (B), (C) in order of slow to fast. These processes should reflect ATP hydrolysis and concomitant energy transduction since they are absent in muscles that are relaxed, in rigor or fixed. The great similarities in the complex stiffness data from different muscles suggests that there is a common mechanism of chemo-mechanical energy transduction across a broad phylogenetic range.

Keywords

Skeletal Muscle Phase Shift Rate Process Physiological Process Common Mechanism 

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Copyright information

© Chapman and Hall Ltd 1980

Authors and Affiliations

  • Masataka Kawai
    • 1
  • Philip W. Brandt
    • 1
  1. 1.Muscle Physiology Division, Departments of Neurology and AnatomyColumbia UniversityNew YorkUSA

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