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Retinoids and a retinoic acid receptor differentially modulate thymosinβ 10 gene expression in transfected neuroblastoma cells

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Summary

  1. 1.

    Investigations have demonstrated that the gene encoding thymosinβ 10 (a 43-amino acid member of a family of related proteins originally described in the rat immune system) is a target for morphogenic retinoids in both human and rat neuroblastoma cells.

  2. 2.

    Structure-activity studies revealed that the stimulatory actions of retinoids upon the thymosinβ 10 gene reflect the differing affinities of retinoid analogues for a retinoic acid receptor.

  3. 3.

    To examine further the possibility that the trophic actions of retinoic acid upon expression of the thymosinβ 10 gene involved retinoid receptors, neuroblastoma cells were transiently transfected with an expression vector encoding the nuclear retinoic acid receptor (α) protein.

  4. 4.

    Northern blot and slot-blot analyses revealed that neuronal cells overexpressing RARα-mRNA exhibited an enhanced sensitivity to exogenous and endogeneous retinoic acid in terms of thymosinβ 10 mRNA. Although the RAR-α gene was expressed (at low levels) a priori in these neuroblastoma cells, retinoic acid (2 × 10−7 M for 3 days) slightly stimulated RAR-α-mRNA accumulation.

  5. 5.

    Collectively, these findings indicate the the retinoic acid receptor (α) is regulated by retinoid acid and that the developmentally regulated, retinoidresponsive thymosinβ 10 gene is a target for this nuclear transcription factor in cells derived from the neural crest.

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  1. Department of Surgery, UMDNJ-NJ Medical School, 185 South Orange Avenue, 07103-2757, Newark, New Jersey, USA

    Alan K. Hall

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  1. Alan K. Hall
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Hall, A.K. Retinoids and a retinoic acid receptor differentially modulate thymosinβ 10 gene expression in transfected neuroblastoma cells. Cell Mol Neurobiol 12, 45–58 (1992). https://doi.org/10.1007/BF00711638

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  • DOI: https://doi.org/10.1007/BF00711638

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