Summary
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1.
Protein composition of neuronal nuclei was studied at two stages of brain maturation, i.e., before (embryonic day 16; E16) and after (postnatal day 10; P10) shortening of the nucleosomal repeat length. Glial nuclei were analyzed in parallel as a control.
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Total nuclear or HCl- and 5% perchloric acid (PCA)-soluble proteins were analyzed by different electrophoretic techniques.
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Our results show an increase in the concentration of histone H1° with differentiation, although the H1 class undergoes an overall decrease.
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The chromatin of mature neurons is also enriched in the ubiquinated form of histone H2A (A24), while the high-mobility group (HMG) proteins 1 and 2 seem to decrease slightly relative to core histones.
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5.
Both quantitative and qualitative differences in the abundance of nonhistone proteins relative to histones accompany neuronal terminal differentiation.
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Cestelli, A., Castiglia, D., Di Liegro, C. et al. Qualitative differences in nuclear proteins correlate with neuronal terminal differentiation. Cell Mol Neurobiol 12, 33–43 (1992). https://doi.org/10.1007/BF00711637
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DOI: https://doi.org/10.1007/BF00711637