Summary
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1.
Adenosine transport is subjected to regulation by hormones. Glucocorticoids, sexual steroids, and retinoic acid inhibit adenosine transport in chromaffin cells after a long-term incubation period (24 hr). No effects were observed after a short-term incubation period (10 min).
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2.
The kinetic parameters of transporters were studied. No significant changes were observed for the affinity constant (K m), whose value remains at 1 ± 0.2µM after 24-hr incubation in the presence of these compounds. The maximal velocity (V max) was significantly modified, with a decrease of about 20% in all cases.
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3.
NBTI binding was not modified in its affinity constant or maximal bound capacity (B max) by the presence of these compounds for a 24-hr incubation period. Thus the efficiency of transporters (quotientV max/B max) changed from 10.9 ± 0.08 adenosine molecules transported per transporter per sec in the control cells to 9.1 ± 0.07 in hormone-treated cultured cells.
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4.
The thyroid hormone (T3) significantly increased adenosine transport in a long-term incubation period in chromaffin cells (24 hr). This activatory effect is antagonized by steroid hormones and retinoic acid.
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References
Bradford, M. M. (1976). Rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem. 72248–254.
Ciaranello, R. D. (1978). Regulation of phenylethanolamine-N-methyltransferase synthesis and degradation. I. Regulation by rat adrenal glucocorticoids.Mol. Pharmacol. 14478–489.
Davis, K. D., and Lazar, M. A. (1992). Selective antagonism of thyroid hormone action by retinoic acid.J. Biol. Chem. 267(5):3185–3189.
Delicado, E. G., Sen, R. P., and Miras-Portugal, M. T. (1991). Effects of phorbol esters and secretagogues on the nitrobenzylthioinosine binding to nucleoside transporters and nucleoside uptake in cultured chromaffin cells.Biochem. J. 279651–655.
Durant, S., Duval, D., and Homo, F. (1983). Potentiation by steroids of theβ-adrenergic agent-induced stimulation of cyclic AMP in isolated mouse thymocytes.Biochim. Biophys. Acta 762315–324.
Edelman, I. S. (1975). Mechanism of action of steroid-hormones.J. Steroid Biochem. 6147–159.
Fideu, M. D., and Miras-Portugal, M. T. (1992). Long term regulation of nucleoside transport by thyroid hormone (T3) in cultured chromaffin cells.Neurochem. Res. 17(11):1099–1104.
Gagne, D., Homo, F., and Duval, D. (1980). Steroid-induced inhibition of nucleoside uptake in isolated mouse thymocytes.Biochim. Biophys Acta 60327–35.
Horner, H. C., Munck, A., and Linehard, G. E. (1987). Dexamethasone causes translocation of glucose transporters from the plasma membrane to an intracellular site in human fibroblasts.J. Biol. Chem. 262(36):17696–17702.
Horner, H. C., Packan, D. R., and Sapolsky, M. (1990). Glucocorticoids inhibit glucose transport in cultured hippocampal neurons and glia.Neuroendocrinology 5257–64.
Kauppinen, R. A., and Nicholls, D. G. (1986). Synaptosomal bioenergetics: The role of glycolysis, pyruvate oxidation and responses to hypoglycaemia.Eur. J. Biochem. 158159–165.
Lee, C. W., and Jarvis, S. M. (1988). Nucleoside transport in rat cerebral-cortical synaptosomes. Evidence for two types of nucleoside transporters.Biochem. J. 249557–564.
Marangos, P. J., and Deckert, J. (1987). [3H]Dipyridamole binding to guinea pig brain membranes: Possible heterogeneity of central adenosine uptake sites.J. Neurochem. 481231–1236.
Miras-Portugal, M. T., Rotllan, P., and Aunis, D. (1985). Incorporation of adenosine into nucleotides of chromaffin cells, maintained in primary cultures.Neurochem. Int. 789–93.
Miras-Portugal, M. T., Torres, M., Rotllan, P., and Aunis, D. (1986). Adenosine transport in bovine chromaffin cells in culture.J. Biol. Chem. 2611712–1719.
Miras-Portugal, M. T., Sen, R. P., and Delicado, E. G. (1991). Nucleoside transport in neurons. Regulation by secretagogues and effectors of protein kinases A and C.Nucleosides & Nucleotides 10(5):965–973.
Murray, D. K., Hill, M. E., and Nelson, D. H. (1990). Inhibitory action of sphingosine, sphinganine and dexamethasone on glucose uptake: Studies with hydrogen peroxide and phorbol ester.Life Sci. 461843–1849.
Roeder, L. M., Hopkins, I. B., Kaiser, J. R., Hanukoglu, L., and Tildon, J. T. (1988). Thyroid hormone action on glucose transporter activity in astrocytes.Biochem. Biophys. Res. Commun. 156275–281.
Rotllán, P., and Miras-Portugal, M. T. (1985). Purine nucleotide synthesis in adrenal chromaffin cells.J. Neurochem. 441029–1036.
Sabban, E., Goldstein, M., Bohn, M. C., and Black, I. B. (1982). Development of the adrenergic phenotype: Increase in adrenal messenger RNA coding for phenylethanolamine-N-methyltransferase.Proc. Natl. Acad. Sci. USA 794823–4827.
Sadler, S. E., and Maller, J. L. (1982). Identification of a steroid receptor on the surface of Xenopus oocytes by photoaffinity labeling.J. Biol. Chem. 257355–361.
Sapolsky, R. (1987). Glucocorticoids and hippocampal damage.Trends Neurosci. 10346–349.
Sen, R. P., Delicado, E. G., and Miras-Portugal, M. T. (1990). Effect of forskolin and cyclic AMP analog on adenosine transport in cultured chromaffin cells.Neurochem. Int. 17523–528.
Sen, R. P., Delicado, E. G., Castro, E., and Miras-Portugal, M. T. (1993). Effect of P2y agonists on adenosine transport in cultured chromaffin cells.J. Neurochem. 60 613–619.
Siesjö, B. (1981). Cell damage in the brain: A speculative synthesis.J. Cereb. Blood Flow Metab. 1155–173.
Torres, M., Bader, M. F., Aunis, D., and Miras-Portugal, M. T. (1987). Nerve growth factor effect on adenosine transport in cultured chromaffin cells.J. Neurochem. 48233–235.
Torres, M., Delicado, E. G., and Miras-Portugal, M. T. (1988). Adenosine transporters in chromaffin cells: Subcellular distribution and characterization.Biochim. Biophys. Acta 969111–120.
Torres, M., Fideu, M. D., and Miras-Portugal, M. T. (1990). All nucleoside transporters in bovine chromaffin cells are nitrobenzylthioinosine sensitive.Neurosci. Lett. 112343–347.
Virgin, C. E. Jr., Ha, T. P.-T., Packan, D. R., Tombaugh, G. C., Yang, S. H., Horner, H. C., and Sapolsky, R. M. (1991). Glucocorticoids inhibit glucose transport and glutamate uptake in hippocampal astrocytes: Implications for glucocorticoid neurotoxicity.J. Neurochem. 571422–1428.
Wu, P. H., and Phillips, J. W. (1984). Uptake by central nervous tissues as a mechanism for the regulation of extracellular adenosine concentrations.Neurochem. Int. 6613–632.
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Fideu, M.D., Miras-Portugal, M.T. Steroid-induced inhibition of adenosine transport in cultured chromaffin cells. Cell Mol Neurobiol 13, 493–502 (1993). https://doi.org/10.1007/BF00711458
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DOI: https://doi.org/10.1007/BF00711458