Skip to main content
SpringerLink
Log in

Practical guide for morphometry of human peroxisomes on electron micrographs

  • Published:
Journal of Inherited Metabolic Disease

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Abe K, Matsushima S, Mita M (1983) Estimation of the mean size of spheres from the profiles in ultrathin sections: a stereological method.J Electron Microsc 32: 57–60.

    Google Scholar 

  • Aubourg P, Scotto J, Rocchiccioli F, Feldmann-Pautrat D, Robain O (1986) Neonatal adrenoleukodystrophy.J Neurol Neurosurg Psychiatry 49: 77–86.

    Google Scholar 

  • Beard ME, Moser AB, Sapirstein V, Holtzman E (1986) Peroxisomes in infantile phytanic acid storage disease: a cytochemical study of skin fibroblasts.J Inher Metab Dis 9: 321–334.

    Google Scholar 

  • De Craemer D (1994) Peroxisomes in acquired and congenital diseases: human and experimental studies. PhD thesis. Brussels, 133–155.

  • De Craemer D, Rickaert F, Wanders RJA, Roels F (1989) Hepatic peroxisomes are smaller in primary hyperoxaluria type 1.Micron Microsc Acta 20: 125–126.

    Google Scholar 

  • De Craemer D, Kerckaert I, Roels F (1991a) Hepatocellular peroxisomes in human alcoholic and drug-induced hepatitis: a quantitative study.Hepatology 14(5): 811–817.

    Google Scholar 

  • De Craemer D, Zweens MJ, Lyonnet S, et al (1991b) Very large peroxisomes in distinct peroxisomal disorders (rhizomelic chondrodysplasia punctata and acyl-CoA oxidase deficiency): novel data.Virchows Archiv(A) 419: 523–525.

    Google Scholar 

  • De Craemer D, Pauwels M, Roels F (1993a) Peroxisomes in cirrhosis of the human liver: a cytochemical, ultrastructural and quantitative study.Hepatology 17: 404–410.

    Google Scholar 

  • De Craemer D, Pauwels M, Hautekeete M, Roels F (1993b) Alterations of hepatocellular peroxisomes in patients with cancer. Catalase cytochemistry and morphometry.Cancer 71(12): 3851–3858.

    Google Scholar 

  • Espeel M, Roels F, Van Maldergem L, et al (1991) Peroxisomal localization of the immunoreactive β-oxidation enzymes in a neonate with a β-oxidation defect: pathological observations in liver, adrenal cortex and kidney.Virchows Archiv(A) 419: 301–308.

    Google Scholar 

  • Espeel M, Heikoop JC, Smeitink JAM, et al (1993) Cytoplasmic catalase and ghost-like peroxisomes in the liver from a child with atypical chondrodysplasia punctata.Ultrastruct Pathol 17: 623–626.

    Google Scholar 

  • Espeel M, Mandel H, Poggi F, et al (1995a) Peroxisome mosaicism in the liver of peroxisomal deficiency patients.Hepatology,22 497–504.

    Google Scholar 

  • Espeel M, Roels F, Giros M, et al (1995b) Immunolocalization of a 43 kDa peroxisomal membrane protein in the liver of patients with generalized peroxisomal disorders.Eur J Cell Biol 67 319–327.

    Google Scholar 

  • Fringes B, Reith A (1982) Time course of peroxisome biogenesis during adaptation to mild hyperthyroidism in rat liver. A morphometric-stereologic study by electron microscopy.Lab Invest 47: 19–26.

    Google Scholar 

  • Goldfischer S, Collins J, Rapin I, et al (1985) Peroxisomal defects in neonatal-onset and X-linked adrenoleukodystrophies.Science 227: 67–70.

    Google Scholar 

  • Goldfischer S, Collins J, Rapin I, et al (1986) Pseudo-Zellweger syndrome: deficiencies in several peroxisomal oxidative activities.J Pediatr 108: 25–32.

    Google Scholar 

  • Hughes JL, Poulos A (1992) Ultrastructural examination of tissues from patiens with peroxisomal disorders. In: Megias-Megias L, Rodriguez-Garcia MI, Rios A, Arias JM, eds.Electron Microscopy 92: Biological Sciences. Granada: University of Granada, 521–522.

    Google Scholar 

  • Hughes JL, Poulos A, Robertson E, et al (1990) Pathology of hepatic peroxisomes and mitochondria in patients with peroxisomal disorders.Virchows Archiv(A) 416: 255–264.

    Google Scholar 

  • Hughes JL, Bourne AJ, Poulos A (1993) Establishment of a normal range of morphometric values for peroxisomes in paediatric liver.Virchow's Archiv(A) 423: 453–457.

    Google Scholar 

  • Kerckaert I, Claeys A, Just W, Cornelis A, Roels F (1989) Automated image analysis of rat liver peroxisomes after treatment with thyroid hormones: changes in number, size and catalase reaction.Micron Microsc Acta 20(1): 9–18.

    Google Scholar 

  • Loud AV (1968) A quantitative stereological description of the ultrastructure of normal rat liver parenchymal cells.J Cell Biol 37: 27–46.

    Google Scholar 

  • Mandel H, Espeel M, Roels F, et al (1994) A new type of peroxisomal disorder with variable expression in liver and fibroblasts.J Pediatr 125: 549–555.

    Google Scholar 

  • Poll-The BT, Saudubray JM, Ogier HAM, et al (1987) Infantile Refsum disease: an inherited peroxisomal disorder. Comparison with Zellweger syndrome and neonatal adrenoleukodystrophy.Eur J Pediatr 146: 477–483.

    Google Scholar 

  • Poll-The BT, Roels F, Ogier H, et al (1988) A new peroxisomal disorder with enlarged peroxisomes and a specific deficiency of acyl-CoA oxidase (pseudo-neonatal adrenoleukodystrophy).Am J Hum Genet 42: 422–434.

    Google Scholar 

  • Roels F (1991) Peroxisomes, a personal account. Brussels: VUB Press, 1–151. ISBN: 90-70289-94-6.

    Google Scholar 

  • Roels F, Cornelis A (1989) Heterogeneity of catalase staining in human hepatocellular peroxisomes.J Histochem Cytochem 37: 331–337.

    Google Scholar 

  • Roels F, Pauwels M, Cornelis A, et al (1983) Peroxisomes (microbodies) in human liver. Cytochemical and quantitative studies of 85 biopsies.J Histochem Cytochem 31: 235–237.

    Google Scholar 

  • Roels F, Cornelis A, Poll-The BT, et al (1986) Hepatic peroxisomes are deficient in infantile Refsum disease: a cytochemical study of 4 cases.Am J Med Genet 25: 257–271.

    Google Scholar 

  • Roels F, Pauwels M, Poll-The BT, et al (1988) Hepatic peroxisomes in adrenoleukodystrophy and related syndromes. Cytochemical and morphometric data.Virchows Archiv(A) 414: 275–285.

    Google Scholar 

  • Roels F, Espeel M, De Craemer D (1991) Liver pathology and immunocytochemistry in congenital peroxisomal diseases: a review.J Inher Metab Dis 14: 853–874.

    Google Scholar 

  • Roels F, Espeel M, Poggi F, Mandel H, Van Maldergem L, Saudubray JM (1993) Human liver pathology in peroxisomal diseases: a review including novel data.Biochimie 75: 281–292.

    Google Scholar 

  • Roels F, Espeel M, Mandel H, et al (1994) Cell and tissue heterogeneity in peroxisomal patients. In Wanders RJA, Schutgens RBH, Tabak HF, eds.Peroxisomal Disorders in Relation to Functions and Biogenesis of Peroxisomes. Amsterdam; Elsevier, in press.

    Google Scholar 

  • Scotto JM, Hadchouel M, Odièvre M, et al (1982) Infantile phytanic acid storage disease, a possible variant of Refsum's disease: three cases including ultrastructural studies of the liver.J Inher Metab Dis 5: 83–90.

    Google Scholar 

  • Smeitink JAM, Beemer FA, Espeel M, et al (1992) Bone dysplasia associated with phytanic acid accumulation and deficient plasmalogen synthesis: a peroxisomal entity amenable to plasmapheresis.J Inher Metab Dis 15: 377–380.

    Google Scholar 

  • Vamecq J, Draye JP, Van Hoof F, et al (1986) Multiple peroxisomal enzymatic deficiency disorders. A comparative biochemical and morphologic study of Zellweger cerebrohepatorenal syndrome and neonatal adrenoleukodystrophy.Am J Pathol 125: 524–535.

    Google Scholar 

  • Van Maldergem L, Espeel M, Wanders RJA, et al (1992) Neonatal seizures and severe hypotonia in a male infant suffering a defect in peroxisomal β-oxidation.Neuromusc Disord 2: 217–224.

    Google Scholar 

  • Weibel ER (1969) Stereological principles for morphometry in electron microscopic cytology.Int Rev Cytol 26: 235–302.

    Google Scholar 

  • Weibel ER (1979)Stereological Methods, vol. 1,Practical Methods for Biological Morphometry. London: Academic Press.

    Google Scholar 

  • Weibel ER, Kistler GS, Scherle WF (1966) Practical stereological methods for morphometric cytology.J Cell Biol 30: 23–38.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Anatomy, Embryology and Histology, University of Gent, Belgium

    I. Kerckaert, D. De Craemer & G. Van Limbergen

  2. Department of Anatomy, Free University of Brussels, Belgium

    D. De Craemer

Authors
  1. I. Kerckaert
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. De Craemer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Van Limbergen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kerckaert, I., De Craemer, D. & Van Limbergen, G. Practical guide for morphometry of human peroxisomes on electron micrographs. J Inherit Metab Dis 18 (Suppl 1), 172–180 (1995). https://doi.org/10.1007/BF00711438

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00711438

Keywords

Search

Navigation