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Immunoblot analysis of peroxisomal proteins in liver and fibroblasts from patients

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Journal of Inherited Metabolic Disease

Summary

Identification of a patient as suffering from a peroxisomal disorder usually starts by the finding of elevated very long-chain fatty acids in plasma and/or serum. This is followed by more detailed studies in blood, fibroblasts and tissues, including immunoblot analysis. Indeed, immunoblot analysis has become a valuable tool in the correct diagnosis and assignment of individual patients, except for X-linked adrenoleukodystrophy (X-ALD). We describe a simple immunoblotting procedure applicable to liver and fibroblast homo-genates using antibodies raised against catalase and the three β-oxidation enzyme proteins acyl-CoA oxidase I, bifunctional protein and peroxisomal thiolase. The same procedure can also be used for chorionic villus biopsy specimens and has now become the method of choice for the prenatal diagnosis of Zellweger syndrome (and other disorders of peroxisome biogenesis) and rhizomelic chondrodysplasia punctata.

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Authors and Affiliations

  1. Academic Medical Centre, Departments of Pediatrics and Clinical Chemistry, Laboratory of Pediatric Clinical Chemistry, Section for Clinical Enzymology, University Hospital Amsterdam, Meibergdreef 9, PO Box 22700, 1100 DE, Amsterdam, The Netherlands

    R. J. A. Wanders, C. Dekker, R. Ofman, R. B. H. Schutgens & P. Mooijer

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  1. R. J. A. Wanders
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  2. C. Dekker
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  3. R. Ofman
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  4. R. B. H. Schutgens
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  5. P. Mooijer
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Wanders, R.J.A., Dekker, C., Ofman, R. et al. Immunoblot analysis of peroxisomal proteins in liver and fibroblasts from patients. J Inherit Metab Dis 18 (Suppl 1), 101–112 (1995). https://doi.org/10.1007/BF00711433

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