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Cellular and Molecular Neurobiology

, Volume 7, Issue 3, pp 317–322 | Cite as

Maitotoxin stimulates phosphoinositide breakdown in neuroblastoma hybrid NCB-20 cells

  • Fabian Gusovsky
  • Takashi Yasumoto
  • John W. Daly
Short Communication

Summary

  1. 1.

    Maitotoxin (MTX) was an extraordinarily potent stimulant of phosphoinositide breakdown in the neuroblastoma hybrid NCB-20 cells.

     
  2. 2.

    Maximal responses were obtained at 0.25–0.5 ng MTX/ml, and resulted in increased formation of [3H]inositol mono-, bis-, and trisphosphates. Increased formation of [3H]inositol bis- and trisphosphate was observed as early as 15 sec after the addition of MTX.

     
  3. 3.

    MTX-induced phosphoinositide breakdown in NCB-20 cells was not antagonized by organic (nifedipine, methoxyverapamil) or inorganic (Mn2+, Co2+, Cd2+) calcium channel blockers. However, the response on phosphoinositide breakdown was completely eliminated in the absence of extracellular calcium.

     
  4. 4.

    The results suggest that MTX either directly stimulates phosphoinositide breakdown in a calcium-dependent manner or acts indirectly through calcium channels insensitive to organic/inorganic calcium channel blockers.

     

Key words

maitotoxin NCB-20 cells calcium channels phosphoinositide breakdown 

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References

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Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • Fabian Gusovsky
    • 1
  • Takashi Yasumoto
    • 2
  • John W. Daly
    • 1
  1. 1.Laboratory of Bioorganic ChemistryNIDDK, National Institutes of HealthBethesdaUSA
  2. 2.Faculty of AgricultureTohoku UniversitySendaiJapan

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