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Cellular and Molecular Neurobiology

, Volume 10, Issue 3, pp 435–445 | Cite as

Regulation of c-fos and c-jun expression in the rat supraoptic nucleus

  • D. A. Carter
  • D. Murphy
Article

Summary

  1. 1.

    We have investigated induction of the nuclear proto-oncogenes c-fos and c-jun in the rat supraoptic nucleus (SON) during physiological stimulation.

     
  2. 2.

    Dehydration (0-24 hr) was asssociated with modest, but significant increases in both c-fos and c-jun mRNA at 8 hr and 16 hr as determined by Northern analysis of total RNA extracted from microdissected SON. Prior to 8 hr, and beyond 24 hr, no consistent changes in c-fos and c-jun mRNA were found. Levels of c-fos and c-jun mRNA in the hippocampus were not altered over 24 hr of dehydration.

     
  3. 3.

    Acute stimulation with hypertonic saline (1.5M, i.p.) resulted in a marked increase in SON c-fos mRNA at 1 hr (6-fold) and 2 hr (3.5-fold). Small increases in SON c-jun mRNA were observed at these time points. Treatment with a similar volume of 0.9% saline did not elevate SON c-fos and c-jun mRNA levels.

     
  4. 4.

    Analysis of transcriptional activity with a nuclear run-on assay showed that activation of transcription appears to mediate the induction of c-fos and c-jun mRNA following acute hypertonic saline treatment. During dehydration transcriptional activation is apparent for c-jun but is not well defined for c-fos.

     
  5. 5.

    The results are discussed with reference to the hypothesis that products of c-fos and c-jun may mediate adaptive changes in hypothalamic gene expression.

     

Key words

c-fos c-jun supraoptic nucleus osmolality transcription mRNA 

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Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • D. A. Carter
    • 1
  • D. Murphy
    • 1
  1. 1.Neuropeptide Laboratory, Institute of Molecular and Cell BiologyNational University of Singapore, Kent RidgeSingaporeSingapore

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