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Chromosome Research

, Volume 3, Issue 1, pp 69–72 | Cite as

Detecting ‘hidden’ exchange events within X-ray-induced aberrations using multicolour chromosome paints

  • P. J. Simpson
  • J. R. K. Savage
Short Communication

Abstract

In a recent series of papers we pointed out that any complex exchange (an interaction involving three or more breaks) can yield a wide range of aberration configurations. If these aberrations are detected with asingle chromosome paint and total centromere probe, the majority will be scored as either ‘simple’ exchanges,apparently derived from two breaks, which we termpseudosimples, or as complexes which appear to have fewer breaks than were actually involved, which we termreduced complexes. In this study, metaphases from X-irradiated human fibroblasts were hybridized to two distinctly labelled composite probes (paints) for chromosomes 1 and 2. All centromeres were marked by differential 4′,6-diamidino-2-phenylindole (DAPI) staining. Aberrations derived from exchanges involving both paints were characterized. With this method, we are able to identify some of the additional exchange events, so confirming the existence of these two classes of aberration. Ultimately we believe it can be used to estimate their frequency.

Key words

chromosome exchanges FISH X-rays 

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References

  1. Brown JM, Kovacs MS (1993) Vizualisation of non-reciprocal chromosome exchanges in irradiated human fibroblasts by fluorescent in situ hybridisation.Radiat Res 136: 71–76.Google Scholar
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Copyright information

© Rapid Communications of Oxford Ltd 1995

Authors and Affiliations

  1. 1.the MRC Radiobiology UnitChromosome Damage GroupChilton, DidcotUK

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