Summary
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1.
In the present investigation we demonstrate the occurrence and immunocytochemical localization of 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; EC 3.1.4.37) in primary nerve cell cultures from rat brain.
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2.
Using a specific antiserum to purified CNPase the enzyme has been detected by immunofluorescence staining on the cell surface as well as within the cell body and processes of oligodendrocytes.
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3.
Substantial amounts of CNPase activity are expressed in these cells prior to the onset of myelin formation.
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4.
The enzyme activity rapidly increases severalfold in culture during developmental stages corresponding to the period around birth.
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5.
In contrast to neuronal tumor cell lines [Müller, H. W.,et al. (1981).J. Neurochem. 37:947–955; Müller, H. W., and Seifert, W. (1982).Cell. Mol. Neurobiol. 2:227–239], primary neurons from rat brain do not synthesize the enzyme.
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Müller, H.W., Seifert, W. Activity and immunocytochemical localization of 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) in primary nerve cell cultures from rat brain. Cell Mol Neurobiol 2, 241–247 (1982). https://doi.org/10.1007/BF00711151
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DOI: https://doi.org/10.1007/BF00711151