Summary
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1.
An antiserum to cholinergic synaptic vesicles fromTorpedo marmorata which recognizes the heparan-like glycosaminoglycan present in these vesicles and reacts specifically with peripheral and presumed central cholinergic nerve terminals in mammalian species has been tested with retinas from a variety of species.
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2.
The antiserum recognizes specific sites in the inner and outer plexiform layers of retinas of rat, ox and frog.
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3.
The distribution is similar to that reported forα-bungarotoxin, a reagent specific for acetylcholine receptors.
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4.
Immunoreactive material was absent from retinal cell bodies.
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5.
Its appearance in development in the two layers coincided with the appearance of synapses in these layers.
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6.
We conclude that the antigenic sites responding to the anti-vesicle anti-serum are associated with the cholinergic endings in the retina.
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Osborne, N.N., Beale, R., Nicholas, D. et al. An antiserum to cholinergic synaptic vesicles fromTorpedo recognizes nerve terminals in retinas from a variety of species. Cell Mol Neurobiol 2, 157–163 (1982). https://doi.org/10.1007/BF00711080
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DOI: https://doi.org/10.1007/BF00711080