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Cellular and Molecular Neurobiology

, Volume 2, Issue 2, pp 105–113 | Cite as

Differential distribution of gangliosides in nerve-cell cultures

  • Wilfried Dimpfel
Article

Summary

  1. 1.

    The continuous tumor-cell lines Neuro 2a, NB41/A3, and C6 were compared with regard to ganglioside synthesis. In the two neuroblastoma lines a very high rate of synthesis of GM2 could be observed, whereas the glioma line C6 showed a high prevalence of synthesizing GM3. None of them produced GD1b or GT1b. [For nomenclature, see the proposal of Svennerholm (1963).]

     
  2. 2.

    Primary cultures appeared to be very similar to nervous tissuein vivo, containing essentially all the major brain gangliosides in comparable amounts.

     
  3. 3.

    Treatment of primary cultures with the neurotoxins kainic acid and hydroxydopamine showed a decrease in neurons as measured by125I-labeled tetanus toxin binding concomitant with a selective loss of GT1b, GD1b, GD1a, and GM1 but not GM3.

     
  4. 4.

    It is concluded that the ganglioside GM3 is confined mainly to nonneuronal cells, whereas the other four gangliosides mentioned are normal constituents of the neuronal membrane.

     

Key words

gangliosides nerve cells cell culture neurotoxins 

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Copyright information

© Plenum Publishing Corporation 1982

Authors and Affiliations

  • Wilfried Dimpfel
    • 1
  1. 1.Pharmakologisches Institut der Justus-Liebig-Universität GieβenGieβenGermany

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