The effects of short-chain fatty acids on the neuronal membrane functions ofHelix pomatia. II. cholinoreceptive properties
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We have examined the effects of short-chain fatty acids on acetylcholine (ACh)-induced transmembrane currents using internally dialized neurons ofHelix.
Decenoic acid, which increased the fluidity of excitable membranes, caused dramatic changes in the voltage sensitivity of ACh currents consisting of an ACh-induced increase in membrane permeability for K+ and Na+ ions and a shift of theErev of these ACh responses to more positive potentials. Valeric acid, which did not change the membrane fluidity, had no effect on this type of ACh response.
Changes of theENa andECl had no effect on the size of the decenoic acid-induced shift of theErev. But the influence of decenoic acid on the voltage sensitivity of ACh-induced currents almost disappeared after the change of theEK by the reduction of the internal K concentration.
Decenoic acid had no effect on ACh responses in which K+ ions were not involved in the generation of ACh-induced currents.
The results suggest that decenoic acid-induced changes in membrane fluidity modulate cholinoreceptive properties of the neuronal membrane by the inhibition of the K+ carrier involved in the generation of ACh responses.
Key wordsmembrane receptor fluidity acetylcholine decenoic acid valeric acid
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