Summary
-
1.
Extracellular recording techniques were used to study the effects of dopamine on postactivation excitability of rat area CA1 hippocampal neurons maintainedin vitro. Population spikes were elicited by delivery of conditioning and test stimulus pulses to afferent fibers. The interval between the conditioning and test volley was set to separate delivery of stimuli by 10 to 80 msec. The effect of superfusion or microtopical application of dopamine (DA) on population responses to test stimulus pulses was studied.
-
2.
When paired stimulus volleys, separated by brief intervals (up to 40 msec), were delivered to afferent fibers, paired-pulse suppression (PPS) was indicated by the amplitude of the population spike elicited by the test volley being smaller than that elicited by the conditioning volley. When paired volleys were separated by longer intervals (40 to 80 msec), the response elicited by the test volley was larger in amplitude than that elicited by the conditioning volley, indicating paired-pulse facilitation (PPF).
-
3.
Following exposure to DA, the amplitude of the population response elicited by the conditioning volley was larger than the amplitude before exposure to DA. This effect was long-lasting, enduring for tens of minutes. However, when the amplitude of the conditioning population response was held constant, the PPS was decreased, indicating disinhibition.
-
4.
It is suggested that dopamine produces a long-lasting attenuation of an intervening inhibitory influence onto CA1 pyramidal neurons.
This is a preview of subscription content, log in via an institution to check access.
References
Alger, B. E., and Nicoll, R. A. (1982). Feed-forward dendritic inhibition in rat hippocampal pyramidal cells studiedin vitro.J. Physiol. (Lond.) 328105–123.
Andersen, P., and Lømo, T. (1966). Mode of activiation of hippocampal pyramidal cells by excitatory synapses on dendrites.Exp. Brain Res. 2247–260.
Andersen, P., Eccles, J. C., and Løyning, Y. (1964). Pathway of postsynaptic inhibition in the hippocampus.J. Neurophysiol. 27608–619.
Benardo, L. S., and Prince, D. A. (1982). Dopamine action on hippocampal pyramidal cells.J. Neurosci. 2415–423.
Bischoff, S., Scatton, G., and Korf, J. (1979). Biochemical evidence for a transmitter role of dopamine in the rat hippocampus.Brain Res. 165161–165.
Bischoff, S., Bittiger, H., and Krauss, J. (1980). In vivo [3H]spiperone binding to the rat hippocampal formation: Involvement of dopamine receptors.Eur. J. Pharmacol. 68305–315.
Biscoe, T. J., and Straughan, D. W. (1966). Micro-electrophoretic studies of neurons in the cat hippocampus.J. Physiol. (Lond.) 183341–359.
Creager, R., Dunwiddie, T., and Lynch, G. (1980). Paired-pulse and frequency facilitation in the CA1 region of thein vitro rat hippocampus.J. Physiol. (Lond.) 299409–424.
Curtis, D. R., and Eccles, J. C. (1960). Synaptic action during and after repetitive stimulation.J. Physiol. (Lond.) 150374–398.
Eccles, J. C. (1943). Synaptic potentials and transmission in sympathetic ganglion.J. Physiol. (Lond.) 101465–483.
Fujita, Y., and Sakata, H. (1962). Electrophysiological properties of CA1 and CA2 apical dendrites of rabbit hippocampus.J. Neurophysiol. 25209–222.
Gribkoff, V. K., and Ashe, J. H. (1984). Modulation by dopamine of population responses and cell membrane properties of hippocampal CA1 neuronsin vitro.Brain Res. 292327–338.
Haas, H. L., and Rose, G. (1982). Long-term potentiation of excitatory synaptic transmission in the rat hippocampus: The role of inhibitory processes.J. Physiol. (Lond.) 329541–552.
Ishikawa, K., Ott, T., and McGaugh, J. A. (1982). Evidence for dopamine as a transmitter in dorsal hippocampus.Brain Res. 232222–226.
Kandel, E. R., Spencer, W. A., and Brinley, F. T. (1961). Electrophysiology of hippocampal neurons. I. Sequential invasion and synaptic organization.J. Neurophysiol. 24225–242.
Mueller, A. L., Krik, K. L., Hoffer, B. J., and Dunwiddie, T. V. (1982). Noradrenergic responses in rat hippocampus: Electrophysiological actions of direct and indirect acting sympathomimetics in thein vivo slice.J. Pharm. Exp. Ther. 223599–605.
Richards, C. D. (1972). Potentiation and depression of synaptic transmission in the olfactory cortex of the guinea-pig.J. Physiol. (Lond.) 222209–231.
Scatton, B., Simon, H., Le Moal, M., and Bischoff, S. (1980). Origin of dopaminergic innervation of the rat hippocampal formation.Neurosci. Lett. 18125–131.
Schröder, H., Kammerer, E., and Matthies, H. Biochemical evidence for dopaminergic transmission in the rat hippocampus. InNeuronal Plasticity and Memory Formation (Ajurone Marcan, C., and Matthies, H., eds.). Raven Press, New York, 1982, pp. 367–371.
Schwab, M. E., Javay-Agid, F., and Agid, Y. (1978). Labeled wheat germ agglutinin as a newly, highly sensitive retrograde tracer in the rat brain hippocampal system.Brain Res. 152145–150.
Segal, M. (1981). The action of norepinephrine in the rat hippocampus: Intracellular studies in the slice preparation.Brain Res. 206107–128.
Simon, H., Le Moal, M., and Calas, A. (1979). Efferents and afferents of the ventral tegmental AIO region studied after local injection of [3H]leucine and horseradish peroxidase.Brain Res. 17817–40.
Swanson, L. W., and Hartman, B. K. (1975). The central adrenergic system. An immunofluorescence study of the location of cell bodies and their efferent connections in the rat utilizing dopamine-B-hydroxylase as a marker.J. Comp. Neurol. 163467–506.
Wong, R. K. S., Prince, D. A., and Basbaum, A. I. (1979). Intradendritic recordings from hippocampal neurons.Proc. Natl. Acad. Sci. (USA) 76986–990.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gribkoff, V.K., Ashe, J.H. Modulation by dopamine of population spikes in area CA1 hippocampal neurons elicited by paired stimulus pulses. Cell Mol Neurobiol 4, 177–183 (1984). https://doi.org/10.1007/BF00711003
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00711003