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Cellular and Molecular Neurobiology

, Volume 3, Issue 3, pp 203–212 | Cite as

Effect of histrionicotoxin on ion channels in synaptic and conducting membranes of electroplax ofElectrophorus electricus

  • Eva Bartels-Bernal
  • Edgar Diaz
  • Rosalina Cadena
  • Jorge Ramos
  • John W. Daly
Article

Summary

  1. 1.

    Histrionicotoxin (HTX) at low concentrations of 5–10µM blocks the postsynaptic potential of the electroplax ofElectrophorus electricus.

     
  2. 2.

    At 100-fold higher concentrations, HTX blocks the directly evoked action potentials of the conducting membrane.

     
  3. 3.

    The pH dependence of the blockade by HTX at synaptic channels is different from that at the conducting membrane. At the synapse HTX is more potent at acid pH, while at the conducting membrane it is more potent at basic pH.

     
  4. 4.

    HTX at high concentrations antagonizes the effects of batrachotoxin, indicative of an effect on the batrachotoxin-sensitive sodium channels involved in action potential generation.

     
  5. 5.

    While the effects of HTX on the synaptic channels are concentration, time, and pH dependent, the effects on the channels of the conducting membrane are, in addition, use dependent, suggesting interactions of HTX with the activated forms of these channels.

     

Key words

batrachotoxin histrionicotoxin acetylcholine sodium channels local anesthetics 

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Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • Eva Bartels-Bernal
    • 1
  • Edgar Diaz
    • 2
  • Rosalina Cadena
    • 1
  • Jorge Ramos
    • 3
  • John W. Daly
    • 4
  1. 1.Seccion de Farmacologia, Departimento de Ciencias FisiologicasUniversidad del ValleCaliColombia
  2. 2.Departimento de FisicaUniversidad del ValleCaliColombia
  3. 3.Departimento de BiologiaUniversidad del ValleCaliColombia
  4. 4.Laboratory of Bioorganic ChemistryNational Institute of Arthritis, Diabetes, and Digestive and Kidney DiseasesBethesdaUSA

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