Virchows Archiv A

, Volume 410, Issue 1, pp 65–68 | Cite as

Frequency and diagnostic value of the virus-like filamentous intranuclear inclusions in giant cell tumor of bone, not associated with Paget's disease

A study of 43 cases
  • René Abelanet
  • Monique Daudet-Monsac
  • Saddek Laoussadi
  • Michel Forest
  • Marie-Cécile Vacher-Lavenu


This paper deals with the paramyxovirus-like intranuclear inclusions observed in giant cells tumours of bone (GCTB). Twenty-one (49%) of 43 cases of GCTB (1977–1985), either fresh and/ or cultured, show these ultrastructural inclusions.

Fifty samples of various bone lesions in which giant cell lesions occurred, including aneurysmal cysts, hyperparathyroidism, osteoblastoma, human and rat osteopetrosis, GCT of tendon sheaths, and non skeletal granuloma were used as controls. These, together with 20 samples of normal bone (osteoclasts) did not contain intranuclear or intracytoplasmic viral inclusions.

Key words

Giant-cell Virus-like inclusion Intranuclear inclusion Giant cell tumour of bone Paget's disease of bone Electron microscopy 


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  1. Abelanet R, Forest M, Vacher-Lavenu MC, Carlioz A, Daudet-Monsac M (1982) Inclusions paramyxovirus-like intranucléaires dans les tumeurs à celloles géantes des os. Leur parenté avec celles des ostéoclastes de la Maladie osseuse de Paget. “R” Rev Int Rhum XII:159–166Google Scholar
  2. Aparisi T, Arborgh B, Ericsson JLE (1979) Giant cell tumor of bone. Variations in patterns of appearance of different cell types. Virchows Arch [Pathol Anat] 381:159–178Google Scholar
  3. Basle MF, Fournier JG, Rozenblatt S, Rebel A (1986) Detection of Measles Virus RNA in pagetic bone cells by in situ hybridization. Calcif Tissues Int [Suppl S30] (Abstr 111)Google Scholar
  4. El Labban NG (1984) Ultrastructural study of intranuclear tubulo-filaments in a giant cell tumor of bone in a patient with Paget“s disease. J Oral Pathol 13:650–660Google Scholar
  5. Erlandson RA (1981) In Erlandson RA: Diagnostic transmission electron microscopy of human tumors. The interpretation of submicroscopic structures in human neoplastic cells. Masson Publishing Inc, New York, Paris, pp 77–90Google Scholar
  6. Gherardi G, Lo Cascio V, Bonucci E (1980) Fine structure of nuclei and cytoplasm of osteoclasts in Paget's disease of bone. Histopathology 4:63–74Google Scholar
  7. Giorkey E, Sinkovics JG, Giorkey P (1971) Electron microscopic observations on structure resembling myxovirus in human sarcomas. Cancer 27:1449–1454Google Scholar
  8. Hanaoka H, Friedman B, Mack RP (1971) Ultrastructure and histogenesis of giant cell tumor of bone. Cancer 25:1408–1423Google Scholar
  9. Howatson AF, Fornasier VL (1982) Microfilaments associated with Paget's disease of bone: Comparison with nucleocapsids of measles virus and respiratory syncitial virus. Intervirology 18:150–159Google Scholar
  10. Kuwana K (1967) Electron microscopicstudy on giant cell tumor of bone. Arch Jpn Chir 36:819–847Google Scholar
  11. Le Charpentier Y, Le Charpentier M, Forest M, Daudet-Monsac M, Vacher-Lavenu MC, Louvel A, Sedel L, Abelanet R (1977) Inclusions intranucléaires dans une tumeur osseuse à cellules géantes. Mise en évidence au microscope électronique. Nouv Presse Med 6:259–262Google Scholar
  12. Mills BG, Singer FR, Weiner LP, Holst PA (1979) Long tissue culture of cells from bone affected by Paget's disease. Calcif Tissues Int 29:79–87Google Scholar
  13. Mills BG, Singer FR, Weiner LP, Holst PA (1980) Cell cultures from bone affected by Paget's disease. Arthritis Rheum 23:1115–1119Google Scholar
  14. Morgan-Capner P, Robinson P, Clewley G, Darby A, Pettingale K (1981) Measles antibody in Paget's disease. Lancet 1:733Google Scholar
  15. Rebel A, Malkani K, Basle M (1974) Anomalies nucléaires de la Maladie osseuse de Paget. Nouv Presse Med 3:1299–1301Google Scholar
  16. Rebel A, Basle M, Pouplard A, Kouyomdjian S, Filmon R, Lepatezour A (1980) Viral antigen in osteoclasts from Paget's disease of bone. Lancet (8190) 2:344–346Google Scholar
  17. Rebel A, Basle M (1981) Maladie osseuse de Paget et virus. Ann Pathol 1:21–26Google Scholar
  18. Schajowicz F, Ubios AM, Santini Araujo E, Cabrini L (1985) Virus like intranuclear inclusions in giant cell tumor of bone. Clin Orthop 201:247–250Google Scholar
  19. Singer FR, Mills BG (1983) Evidence for a viral etiology of Paget's disease of bone. Clin Orthop 178:245–251Google Scholar
  20. Thiery JP, Mazabraus A, Voisin MC, Lacroix G (1975) Etude cytologique de la tumeur à cellules géantes. Microscopie électronique. Rev Chir Orthop 61:377–386Google Scholar
  21. Vacher-Lavenu MC, Louvel A, Daudet-Monsac M, Abelanet R (1980) Configuration et position des centrioles des cellules mono et multinucléées des tumeurs à cellules géantes des os longs. Ann Anat Pathol 25:331–339Google Scholar
  22. Vacher-Lavenu MC, Louvel A, Daudet-Monsac M, Le Charpentier Y, Abelanet R (1981) Inclusions tubulo-filamenteuses intranucléaires dans les cellules multinculéées des tumeurs à cellules géantes des os. Etude ultrastructurale d'une série de 31 tumeurs. CR Acad Sci (Paris) 293:639–644Google Scholar
  23. Welsh RA, Mayer AT (1970) Nuclear fragmentations and associated fibrils in giant cell tumor of bone. Lab Invest 22:63–72Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • René Abelanet
    • 1
  • Monique Daudet-Monsac
    • 1
  • Saddek Laoussadi
    • 1
  • Michel Forest
    • 1
  • Marie-Cécile Vacher-Lavenu
    • 1
  1. 1.Service Central d'Anatomie et Cytologie pathologiquesHopital CochinParis, Cedex 14France

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