Virchows Archiv A

, Volume 410, Issue 3, pp 221–229 | Cite as

Biological tissue markers in benign and malignant disease of the human prostate

  • Philippe Chastonay
  • Jean Hurlimann
  • Daniel Gardiol


Immunoperoxidase techniques were used to study, the distribution of peanut agglutinin receptors, blood group isoantigens and several epithelial antigens in hyperplasia, adenosis, microcarcinoma and well differentiated adenocarcinoma of the prostate. Intraluminal and luminal surface PNA receptors were seen in all well differentiated carcinomas, 53% of microcarcinomas and 50% of adenosis, while no such sites could be demonstrated in benign hyperplasia. The expected blood group isoantigen was expressed in 75% of benign hyperplasias. When compared to the hyperplastic epithelium nearby, appropriate ABH expression was seen in 60% of adenosis, 47% of microcarcinomas and 25% of well differentiated carcinomas. A keratin antibody specifically labelling the basal cells in the normal prostate identified a subset of well differentiated carcinomas with preferential staining of the apical cytoplasm while microcarcinomas and adenosis were consistently negative. Our study establishes a highly ordered PNA receptor distribution in prostatic epithelia; it confirms early changes in the expression of ABH isoantigens in epithelial proliferative disorders of the prostate; it identifies a subset of keratin-positive well differentiated carcinomas, possibly of different ontogeny.

Key words

Prostate Keratin ABH-isoantigens Peanut-agglutinin 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Achtstätter T, Moll R, Moore B, Franke WW (1985) Cytokeratin polypeptide patterns of different epithelia of the human male urogenital tract: immunofluorescence and gel electrophoretic studies. J Histochem 35:415–426Google Scholar
  2. Akazaki K, Stemmermann GN (1973) Comparative study of latent carcinoma of the prostate among Japanese in Japan and Hawaii. J Nat Canc Inst 50:1137–1144Google Scholar
  3. Bischof W, Aumüller G (1982) Age dependent changes in the carbohydrate pattern of human prostatic epithelium as determined by peroxidase labelled lectins. Prostate 3:507–513Google Scholar
  4. Brawer MK, Peehl DM, Stamey TA, Bostwick DG (1985) Keratin immunoreactivity in the benign and neoplastic human prostate. Cancer Res 45:3663–3667Google Scholar
  5. Brawn PN (1982) Adenosis of the prostate: A dysplastic lesion that can be confused with prostate adenocarcinoma. Cancer 49:826–833Google Scholar
  6. Brawn PN, Ayala AG, v. Eschenbach AC, Hussey DH, Johnson DE (1982) Histologic grading study of prostate adenocarcinoma: The development of a new system and comparison with other methods — a preliminary study. Cancer 49:525–532Google Scholar
  7. Brown JC, Hunt RC (1978) Lectins. Int Rev Cytol 52:277–349Google Scholar
  8. Davidsohn I (1972) Early immunologic diagnosis and prognosis of carcinoma. Am J Clin Pathol 57:715–730Google Scholar
  9. De Lellis RA, Sternberger LA, Mann RB, Banks PM, Nakane PK (1979) Immunoperoxidase technics in diagnostic pathology: Report of a workshop sponsored by the NCI. Am J Clin Pathol 71:483–488Google Scholar
  10. Dohm G (1979) Frühe neoplastische Veraenderungen der Prostata. Verh Dtsch Ges Pathol 63:218–231Google Scholar
  11. Ernst C, Thurin J, Atkinson B (1984) Monoclonal antibody localization of A and B isoantigens in normal and malignant fixed tissue. Am J Pathol 117:451–461Google Scholar
  12. Franklin WA (1983) Tissue binding of lectins in disorders of the breast. Cancer 51:295–300Google Scholar
  13. George DI, Burzynski NJ, Miller RL (1979) Reaction properties of oral lesions to the specific red cell adherence test. Oral Surg 47:51–57Google Scholar
  14. Gupta RK, Schuster R, Christian WD (1973a) Loss of isoantigens A, B and H in prostate. Am J Pathol 70:439–443Google Scholar
  15. Gupta RK, Schuster R (1973b) Isoantigens A, B and H in benign and malignant lesions of breast. Am J Pathol 72:253–260Google Scholar
  16. Häkkinen I (1970) A-like blood group antigen in gastric cancer cells of patients in blood groups 0 or B. B J Nat Cancer Inst 44:1183–1193Google Scholar
  17. Helpap B (1980) The biological significance of atypical hyperplasia of the prostate. Virchows Arch [Pathol Anat] 387:307–317Google Scholar
  18. Hsu S, Raine L (1982) Versatility of biotin-labeled lectins and avidin-biotin-peroxidase complex for localization of carbohydrate in tissue sections. J Histochem Cytochem 30:157–161Google Scholar
  19. Hurlimann J, Gloor E (1984) Adenocarcinoma in situ and invasive adenocarcinoma of the uterine cervix: a immunohistologic study with antibodies specific for several epithelial markers. Cancer 54:103–109Google Scholar
  20. Kapadia A, Feizi T, Jewell D, Keeling J, Slavin G (1981) Immunocytochemical studies of blood group A, H, I and i antigens in gastric mucosae of infants with normal gastric histology and of patients with gastric carcinoma and chronic benign peptic ulceration. J Clin Pathol 34:320–337Google Scholar
  21. Kastendieck H, Altenähr E (1975) Morphogenese und Bedeutung von Epithelmetabplasien in der menschlichen Prostata. Eine elektronenmikroskopische Studie. Virchows Arch [Pathol Anat] 365:137–150Google Scholar
  22. Kastendieck H (1980) Correlations between atypical primary hyperplasia and carcinoma of the prostate. A histological study of 180 total prostatectomies. Pathol Res Pract 169:366–387Google Scholar
  23. Kern WH (1978) Well differentiated adenocarcinoma of the prostate. Cancer 41:2046–2054Google Scholar
  24. Klein PJ, Vierbuchen M, Wurz H, Schulz KD, Newman RA (1981) Secretion-associated lectin- binding sites as a parameter of hormone dependence in mammary carcinoma. Br J Cancer 44:746–748Google Scholar
  25. Lange PH, Limas C, Fraley EE (1978) Tissue blood-group antigens and prognosis in low stage transitional cell carcinoma of the bladder. J Urol 119:52–55Google Scholar
  26. Leathem A, Atkins N (1983) Lectin binding to formalin fixed paraffin sections. J Clin Pathol 36:747–750Google Scholar
  27. Leathem A, Dokal I, Atkins N (1983) Lectin binding to normal and malignant breast tissue. Diagnostic Histopathology 6:171–180Google Scholar
  28. Lee AK, DeLellis RA, Rosen PP, Tallenberg K, Gangi MD, Wolfe HJ (1984) Evaluation of monoclonal antibodies for the study of ABH blood group isoantigens in breast carcinomas. Lab Invest 50:34AGoogle Scholar
  29. Lloyd RV, Foley J, Judd JW (1984) Peanut lectin agglutinin and α-lactalbumin: Binding and immunohistochemical localization in breast tissues. Arch Pathol Lab Med 108:392–395Google Scholar
  30. Louis CJ, Wyllie RG, Chou ST, Sztynda T (1981) Lectin binding affinities of human epidermal tumors and related conditions. Am J Clin Pathol 75:642–647Google Scholar
  31. Louis CJ, Sztynda T, Cheng ZM, Wyllie RG (1983) Lectin-binding affinities of human breast tumors. Cancer 52:1244–1250Google Scholar
  32. Moore RA (1943) Benign hypertrophy of the prostate. A morphological study. J Urol 50:680–710Google Scholar
  33. Orgad U, Alroy J, Ucci A, Merk FB (1984) Histochemical studies of epithelial cell glycoconjugates in atrophic, metaplastic, hyperplastic and neoplastic canine prostate. Lab Invest 50:294–301Google Scholar
  34. Overbeck v. J, Stähli C, Gudat F Carmann H, Lautenschlager C, Dürmüller U, Takacs B, Miggiano V, Staehlin T, Heitz PU (1985) Immunohistochemical characterization of anti-epithelial monoclonal antibody (mab lu-5). Virchows Arch [Pathol Anat] 407:1–12Google Scholar
  35. Rognum TO, Brandtzaeg P, Orjasaeter H, Elgjo K, Hognestad J (1980) Immunohistochemical study of secretory component, secretory IgA and carcinoembryonic antigen in large bowel carcinomas. Pathol Res Pract 170:126–145Google Scholar
  36. Schlegel R, Banks-Schlegel S, McLeod JA, Pinkus GS (1980a) Immunoperoxidase localization of keratin in human neoplasms. Am J Pathol 101:41–50Google Scholar
  37. Schlegel R, Banks-Schlegel S, Pinkus GS (1980b) Immunohistochemical localization of keratin in normal human tissues. Lab Invest 42:91–96Google Scholar
  38. Slocombe GW, Berry CL, Swettenham KV (1980) The variability of blood group antigens in gastric carcinoma as demonstrated by the immunoperoxidase technique. Virchows Arch [Pathol Anat] 387:289–300Google Scholar
  39. Sun T, Green H (1978) Keratin filaments of cultured human epidermal cells. Formation of intramolecular disulfide bonds during terminal differentiation. J Biol Chem 253:2053–2060Google Scholar
  40. Sun T, Shih C, Green H (1979) Keratin cytoskeletons in epithelial cells of internal organs. Proc Natl Acad Sci USA 76:2813–2817Google Scholar
  41. Szulman AE (1966) Chemistry, distribution and function of blood group substances. Annu Rev Med 17:307–322Google Scholar
  42. Tannenbaum M (1974) Atypical epithelial hyperplasia or carcinoma of the prostate gland: The surgical pathologist at an impasse? Urology 4:758–760Google Scholar
  43. Ucci AA, Alroy J, Orgad U, Goyal V, Gavris V (1983) Distribution of lectin binding sites in normal and pathologic human prostates. Lab Invest 48:87A-88AGoogle Scholar
  44. Walker PD, Karnik S, deKerion JB, Pramberg JC (1984) Cell surface blood group antigens in prostatic carcinoma. AM J Clin Pathol 81:503–506Google Scholar
  45. Whitmore WF (1973) The natural history of prostatic carcinoma. Cancer 32:1104–1112Google Scholar
  46. Wiley EL, Mendelsohn G, Eggleston JC (1981) Distribution of carcinoembryonic antigen and blood group substances in adenocarcinoma of the colon. Lab Invest 44:507–513Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Philippe Chastonay
    • 1
  • Jean Hurlimann
    • 1
  • Daniel Gardiol
    • 1
  1. 1.Department of PathologyUniversity of Lausanne, Medical SchoolLausanneSwitzerland

Personalised recommendations