Chromosome Research

, Volume 3, Issue 2, pp 137–138 | Cite as

Establishment of the marker order pter-NRAS-NGFB-D1S189-D1S252-D1S440-D1S453-D1S514-CEN-D1S442-D1S498-qter in relation to the centromere on human chromosome 1

  • Nigel Hoggard
  • Bill Brintnell
  • Yvonne Hey
  • David Jones
  • Jean Weissenbach
  • Erika Mitchell
  • Jennifer Varley
Short Communications

Abstract

Recent developments in genetic linkage mapping of the human genome have generated a large number of short tandem repeat polymorphic markers (Weissenbachet al. 1992, Gyapayet al. 1994), and eventual integration of these markers into a physical map is a logical progression. A number of Généthon microsatellite (CA repeat) markers have been provisionally localized to 1p13, but their exact position with respect to other sequences is unknown. In order to confirm the order of these markers and their position with respect to known genes within 1p13 and the centromere, we have isolated yeast artificial chromosomes (YACs) corresponding to the markers and have carried out double and triple fluorescencein situ hybridization (FISH) studies. Knowledge of both the order of microsatellite markers and their integration with a physical map of known genes can be an essential component in analysis of disease loci such as human cancer, where regions of chromosomes showing high levels of loss of heterozygosity need to be mapped in detail.

Key words

1p13 gene mapping human chromosome 1 linkage 

References

  1. Anand R, Riley JH, Butler R, Smith JC, Markham AF (1990) A 3.5 genome equivalent multi access YAC library: construction, characterization, screening and storage.Nucleic Acids Res 18: 1951–1956.Google Scholar
  2. Gyapay G, Morissette J, Vignal A.et al. (1994) The 1993–1994 Généthon human genetic linkage map.Nature Genet 7, 246–339.Google Scholar
  3. Mitchell ELD, Jones D, White GRM, Varley JM, Santibanez-Koref MF (1995) Establishment of the gene order cen-CD2-NGFB-NRAS-pter at chromosome band 1p13 by fluorescencein situ hybridisation.Cytogenet Cell Genet, in press.Google Scholar
  4. Weissenbach J, Gyapay G, Dib C.et al. (1992) A second-generation linkage map of the human genome.Nature 359: 794–801.Google Scholar

Copyright information

© Rapid Communications of Oxford Ltd 1995

Authors and Affiliations

  • Nigel Hoggard
    • 1
  • Bill Brintnell
    • 1
  • Yvonne Hey
    • 1
  • David Jones
    • 1
  • Jean Weissenbach
    • 2
  • Erika Mitchell
    • 1
  • Jennifer Varley
    • 1
  1. 1.the CRC Department of Cancer Genetics, Paterson Institute for Cancer ResearchChristie HospitalManchesterUK
  2. 2.GénéthonEvryFrance

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