MULTIPRINS: a method for multicolour primedin situ labelling
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With this fast and simple MULTIPRINS protocol we show simultaneous mapping of multiple repetitive sequences by sequential cycles of primedin situ (PRINS) labelling with various primers and reporter molecules. Differentially labelled DNA sequences synthesizedin situ were visualized by directly incorporated rhodamine-4-dUTP, by fluorescein isothiocyanate and Cy5 using digital imaging microscopy. This further development of the PRINS method enhances its potential as an alternative to traditionalin situ hybridization.
Key wordsfluorescence labelling MULTIPRINS repetitive DNA mapping
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