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Malignant lymphoma of true histiocytic origin: Histiocytic Sarcoma

A morphological, ultrastructural, immunological, cytochemical and clinical study of 10 cases

Virchows Archiv A volume 391, pages 249–265 (1981)Cite this article

Summary

Ten tumors of true histiocytic origin (Histiocytic Sarcoma) are presented. The tumor cells were identified as histiocytes by immunological, cytochemical and ultrastructural criteria (cytoplasmic lysozyme activity, presence of C3 and Feγ receptor, strong acid phosphatase and alpha-naphtyl acetate esterase activity, presence of lysosomes, absence of cell junctions and evidence of phagocytosis). The tumors identified in this way showed the following histological characteristics: diffuse proliferation of large tumor cells with ample cytoplasm, containing granular or occasionally diffuse diastase resistent PAS positive material, erythrophagocytosis, and haemosiderin pigment. The large or enormous nuclei were irregular, with occasional deep indentations, sharply defined nuclear membrane, coarse chromatin and conspicuous nucleoli. Despite the uniformity of these criteria differences in presence of alpha1-antitrypsin, alpha1-antichymotrypsin and 5 Nucleotidase activity and the number of lysosomes in the cytoplasm were found. The findings are suggestive of a spectrum of cytological changes in these Histiocytic Sarcomas.

The clinical picture ranged from monolocalization in a lymphoid organ to that of a diffuse Malignant Histiocytosis. The relationship between good response to therapy and complete remission and the absence of alpha1-antitrypsin and a high number of lysosomes is discussed.

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References

  • Abele DC, Griffin TB (1972) Histiocytic medullary reticulosis. Report of 2 cases and review of the literature. Arch Dermatol 106:319–329

    Google Scholar 

  • Barka T, Anderson PJ (1965) Histochemistry. Theory, practice and bibliography, 2nd edn, Hoeber Medical Division, Harper & Row Publ Inc, New York

    Google Scholar 

  • Bosman FT, Kreuning J, Van der Wal AM. Kuiper G, Lindeman J (1977) The influence of fixation on immunoperoxidase staining of plasma cells in paraffin sections of intestinal biopsy specimens. J Histochem 53: 57–62

    Google Scholar 

  • Boston HC, Dahlin DC, Ivins JC, Cupps RE (1974) Malignant lymphoma (so-called reticulum sarcoma) of bone. Cancer 34:1131–1137

    Google Scholar 

  • Byrne GE, Rappaport H (1973) Malignant histiocytosis. Gann Monograph on Cancer Research 15:145–162

    Google Scholar 

  • Carbone PP, Kaplan HS, Musshoff K, Smithers D, Wand Tubiana M (1971) Report of the committee on Hodgkin's disease staging classification. Cancer Res 31:1860–1861

    Google Scholar 

  • Corrin B, Basset F (1979) A review of histiocytosis X with particular reference to eosinophilic granuloma of the lung. Invest Cell Pathol 2:137–146

    Google Scholar 

  • Elema JD, Poppema S (1978) Infantile histiocytosis X (Letterer-Siwe disease). Investigation with enzyme histochemical and sheep erythrocyte rosetting technique. Cancer 42:555–565

    Google Scholar 

  • Van Furth R (1979) Cells of the mononuclear phagocyte system. Nomenclature in terms of sites and conditions. In: Van Furth (ed) Mononuclear phagocytes. Blackwell Scientific Publ, Oxford

    Google Scholar 

  • Gomori G (1952) Microscopic histochemistry: principle and practice. Chicago University Press, Chicago, p 193

    Google Scholar 

  • Gomori G (1946) The study of enzymes in tissue sections. Am J Clin Pathol 16:347–352

    Google Scholar 

  • Graham RC, Karnowsky MJ (1966) The early stages of absorption of injected horse radish peroxidase, in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302

    Google Scholar 

  • Hijmans W, Schuit HRE, Klein F (1969) An immunofluorescence procedure for the detection of intracellular immunoglobulins. Clin Exp Immunol 4:457–472

    Google Scholar 

  • Isaacson P, Wright DH (1978) Malignant histiocytosis of the intestine. Its relationship to malabsorption and ulcerative jejunitis. Hum Pathol 9:661–677

    Google Scholar 

  • Isaacson P, Jones DB. Judd MA (1979a) α1-Antitrypsine in human macrophages. Lancet 1979:964–965

    Google Scholar 

  • Isaacson P, Wright DH, Judd MA, Mephan BL (1979b) Primary gastrointestinal lymphomas. A classification of 66 cases. Cancer 43:1805–1819

    Google Scholar 

  • Leder L-D, Stutte HJ (1975) Seminär für hämatologisch-zytochemische Techniken. Verh Dtsch Ges Pathol 59:503–509

    Google Scholar 

  • Lennert K (1978) Malignant lymphomas, other than Hodgkin's disease. In: Handbuch der Speziellen Pathologischen Anatomie und Histologie, Erster Band, Dritter Teil, Bandteil B. Springer, Berlin-Heidelberg-New York, pp 456–463

    Google Scholar 

  • Lombardi L, Carbone A, Pilotti S, Rilke F (1978) Malignant histiocytosis: a histological and ultrastructural study of lymph nodes in 6 cases. Histopathology 2:315–328

    Google Scholar 

  • Van der Loo EM, Cornelisse CJ, Van Vloten WA, Van der Velde EA, Scheffer E, Meijer CJLM (1980) Diagnostic morphometry of isolated lymph node cells from patients with mycosis fungoides and Sézary's syndrome. Virchows Arch [Cell Pathol] 33:107–116

    Google Scholar 

  • Lukes RJ, Collins RD (1975) New approaches to the classifications of the lymphomata. Br J Cancer 31:Suppl II, 1–28

    Google Scholar 

  • Mahoney JP, Alexander RW (1980) Primary histiocytic lymphoma of bone. A light and ultrastructural study of 4 cases. Am J Surg Pathol 4:149–161

    Google Scholar 

  • Mason DY, Taylor CR (1975) The distribution of muramidase (lysozyme) in human tissue. J Clin Pathol 28:124–132

    Google Scholar 

  • Meijer CJLM, Lindeman J (1975) A modified method for tissue localization of cells bearing a complement receptor. J Immunol Methods 9:59–65

    Google Scholar 

  • Meijer CJLM, Van de Putte, LBA, Eulderink F, Kleinjan R, Lafeber G, Bots GThAM (1977) Characteristics of mononuclear cell populations in chronically inflamed synovial membranes. J Pathol 121:1–8

    Google Scholar 

  • Meijer CJLM, Van der Loo EM, Van Vloten WA, Van der Velde EA, Scheffer E, Cornelisse CJ (1980) Early diagnosis of mycosis fungoides and Sézary's syndrome by morphometric analysis of lymphoid cells in the skin. Cancer 45:2864–2871

    Google Scholar 

  • Meister P, Huhn D, Nathrath W (1980a) Malignant histiocytosis. Immunohistochemical characterization on paraffin embedded tissue. Virchows Arch [Pathol Anat] 385:233–246

    Google Scholar 

  • Meister P, Nathrath W (1980b) Immunohistochemical markers of histiocytic tumors. Human Pathol 11:300–301

    Google Scholar 

  • Mendelsohn G, Eggleston JC, Mann RB (1980) Relationship of lysozyme (muramidase) to histiocytic differentiation in malignant histiocytosis. Cancer 45:273–279

    Google Scholar 

  • Rausch PG, Herion JC, Carney CN, Weinstein P (1979) Malignant histiocytosis. A cytochemical and electron microscopic study of an unusual case. Cancer 44:2158–2164

    Google Scholar 

  • Rosai J, Dorfman RF (1969) Sinus histiocytosis with massive lymphadenopathy. A newly recognized benign clinicopathological entity. Arch Pathol 87:63–70

    Google Scholar 

  • Scott RB, Robb-Smith AHT (1939) Histiocytic modullary reticulosis. Lancet ii: 194–198

    Google Scholar 

  • Sternberger LA (1977) Immunocytochemistry. Prentice-Hall Inc, London, pp 53–54

    Google Scholar 

  • Taylor CR, Mason DY (1974) The immunohistological detection of intracellular immunoglobulins in formalin-paraffin sections from multiple myeloma and related conditions, using the immunoperoxidase technique. Clin Exp Immunol 18:417–429

    Google Scholar 

  • Tubbs RE, Savage RA, Crabtree RH, Sebek BA, Valenzuela R, Benjamin SP (1979) Expression of monocytic-histiocytic cytochemical markers in epithelial neoplasia. Am J Clin Pathol 72:789–794

    Google Scholar 

  • Te Velde J, Burkhardt R, Kleiverda K, Leenheers-Binnendijk L, Sommerfield W (1977) Methylmethacrylate as an embedding medium in histopathology. Histopathology 1:319–330

    Google Scholar 

  • Wachstein M, Meisel E (1957) Histochemistry of hepatic phosphatases at a physiological pH. With a special reference to the demonstration of bile canaliculi. Am J Clin Pathol 27:13–23

    Google Scholar 

  • Weiss SW, Enzinger FM (1978) Malignant fibrous histiocytoma. A clinicopathological study of 200 cases. Cancer 41:2250–2266

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology of the University Medical Centre Leiden, The Netherlands

    P. van der Valk, J. te Velde, D. J. Ruiter, P. J. Spaander, C. J. Cornelisse & C. J. L. M. Meijer

  2. Department of Internal Medicine of the University Medical Centre Leiden, The Netherlands

    J. Jansen

Authors
  1. P. van der Valk
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  2. J. te Velde
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  3. J. Jansen
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  4. D. J. Ruiter
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  5. P. J. Spaander
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  6. C. J. Cornelisse
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  7. C. J. L. M. Meijer
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van der Valk, P., te Velde, J., Jansen, J. et al. Malignant lymphoma of true histiocytic origin: Histiocytic Sarcoma. Virchows Arch. A Path. Anat. and Histol. 391, 249–265 (1981). https://doi.org/10.1007/BF00709158

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  • DOI: https://doi.org/10.1007/BF00709158

Key words

  • Histiocytic sarcoma
  • Histology
  • Marker studies
  • Morphometry
  • Clinical behavior