Virchows Archiv A

, Volume 409, Issue 3, pp 385–393 | Cite as

The sinusoidal barrier in alcoholic patients without liver fibrosis

A morphometric study
  • F. Sztark
  • P. Latry
  • A. Quinton
  • C. Balabaud
  • P. Bioulac-Sage
Article

Summary

Alcohol induces morphological changes in the endothelial and perisinusoidal cells at the fibrotic stage of alcoholic liver diseases. Directly or indirectly, through hemodynamic disturbances linked to the enlargement of steatotic hepatocytes, alcohol may modify this barrier before the onset of fibrosis. Liver biopsies were obtained from control and from alcoholic patients and perfusion-fixed. Volume and surface densities of endothelial cells, perisinusoidal cells and their processes were measured. Liver histology was normal in the 2 groups except for steatosis in the alcoholics. Volume densities represented 8.2%, 4.7% and 3.2% of the sinusoid in controls for endothelial cells, perisinusoidal cells and their processes whereas surface densities represented respectively 0.5, 0.23, 0.21 m2/cm3 of sinusoid. Morphometric values were not significantly different in the alcoholic patients. In none of the alcoholic patients did fine morphological studies of sinusoidal cells give any indication of the possible evolution of the alcoholic disease towards fibrosis. These results indicate that in the group of patients studied, alcohol, before the fibrotic stage, did not significantly alter the sinusoidal barrier.

Key words

Alcohol Human Sinusoidal cells Morphometry 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Blouin A, Bolender RP, Weibel ER (1977) Distribution of organelles and membranes between hepatocytes and non hepatocytes in the rat liver parenchyma. J Cell Biol 72:441–445Google Scholar
  2. Bioulac-Sage P, Balabaud C, Dubroca J, Boussarie L, Grimaud JA, Latry P, Lamouliatte H, Quinton A (1986) Sinusoids and the Disse space in patients with liver diseases. In: Laurent P, Grimaud JA, Bienvenu J (eds) Marker proteins in inflammation. Walter de Gruyter and Co, BerlinGoogle Scholar
  3. Fraser R, Bowler LM, Day WA, Dobbs B, Johnson HD, Lee D (1980) High perfusion pressure damages the sieving of sinusoidal endothelium in rat livers. Br J Exp Pathol 61:222–228Google Scholar
  4. Fraser R, Bowler LM, Day WA (1980) Damage of rat liver sinusoidal endothelium by ethanol. Pathology 12:371–376Google Scholar
  5. Junqueira LCU, Bignolas G, Brentani RR (1979) A simple and sensitive method for the quantitative estimation of collagen. Anal Biochem 94:96–99Google Scholar
  6. Mak K, Lieber CS (1984) Alterations in endothelial fenestrations in liver sinusoids of baboons fed alcohol: a scanning electron microscopic study. Hepatology 4:386–391Google Scholar
  7. Mak K, Leo MA, Lieber CS (1984) Alcoholic liver injury in baboons, transformation of lipocytes to transitional cells. Gastroenterology 87:188–200Google Scholar
  8. Miyakawa H, Iida S, Leo MA, Greenstein RJ, Zimmon DS, Lieber CS (1985) Pathogenesis of precirrhotic portal hypertension in alcohol-fed baboons. Gastroenterology 88:143–150Google Scholar
  9. Nopanitaya W, Lamb JC, Grisham JW, Carson JL (1976) Effect of hepatic venous outflow obstruction on pores and fenestrations in sinusoidal endothelium. Br J Exp Pathol 57:604–609Google Scholar
  10. Vidins EI, Britton RS, Medline A, Blendis LM, Israel Y, Orrego H (1985) Sinusoidal caliber in alcoholic and nonalcoholic liver disease: diagnostic and pathogenic implications. Hepatology 5:408–414Google Scholar
  11. Weibel ER, Kistler GS, Scherle WF (1966) Practical stereological methods for morphometric cytology. J Cell Biol 30:23–38Google Scholar
  12. Wisse E, De Zanger RB, Charels K, Van der Smissen P, McCuskey RS (1985) The liver sieve: considerations concerning the structure and function of endothelial fenestrae, the sinusoidal wall and the space of Disse. Hepatology 5:683–692Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • F. Sztark
    • 2
  • P. Latry
    • 1
    • 2
  • A. Quinton
    • 1
  • C. Balabaud
    • 1
    • 2
  • P. Bioulac-Sage
    • 2
  1. 1.Service des maladies de l'appareil digestifHôpital Saint AndréBordeauxFrance
  2. 2.Laboratoire des Intéractions CellulairesUniversité de Bordeaux IIBordeauxFrance

Personalised recommendations