Summary
The incidence and significance of the expression of the antigen defined by the monoclonal antibody Ca 19.9 (Sialyl Lea) has been assessed in human breast tissue. Frozen and formalin-fixed, paraffin embedded specimens of normal, hyperplastic, pregnant breast and carcinomas were examined using an immunoperoxidase technique.
Ductal and acinar epithelium of normal and hyperplastic tissues showed variable reactivity in frozen sections but there was a reduction in staining in comparable samples after fixation and processing, such that in many instances only focal ductal epithelium reacted. A distinctive feature in the pregnant breast was the absence of staining in acini showing differentiated secretory activity, despite a reaction in adjacent non-secretory acini and ducts.
The overall incidence of detection of the Ca 19.9 antigen in breast carcinomas was 62%, but in half of these only a small number of cells stained. A significant relationship between expression of Sialyl Lea and poor differentiation of carcinomas was identified, but there was no correlation with local lymph node status. In contrast to the non-malignant tissue fixation and processing had little effect on the reactivity of carcinomas. It is suggested that this difference may be quantitative in nature, with malignant breast showing much greater expression, or be related to organisation of the antigen.
The observations concerning carcinomas and pregnant breast indicate that the synthesis of the Ca 19.9 antigen is related to the state of differentiation and functional activity of human breast.
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References
Arends JW, Verstynen C, Bosman FT, Hilgers J, Steplewski Z (1983a) Distribution of monoclonal antibody-defined monosialoganglioside in normal and cancerous human tissues: an immunoperoxidase study. Hybridoma 2:219–229
Arends JW, Wiggers T, Schutte B, Thijs CT, Verstynen C, Hilgers J, Blijham GH, Bosman FT (1983b) Monoclonal antibody (1116 NS 19.9) defined monosialoganglioside (GICA) in colorectal carcinoma in relation to stage, histopathology and DNA flow cytometry. Int J Cancer 32:219–229
Atkinson B, Ernst CS, Herlyn M, Steplewski Z, Sears HF, Koprowski H (1982) Gastrointestinal cancer-associated antigen in immunoperoxidase assay. Cancer Res 42:4820–4822
Blaszczyk M, Hansson GC, Karlsson KA, Larson G, Stromberg N, Thurin J, Herlyn M, Steplewski Z, Koprowski H (1984) Lewis blood group antigens defined by monoclonal anti-colon carcinoma antibodies. Arch Biochem Biophys 233:161–168
Elston CW, Gresham GA, Rao GS, Zebro T, Haybittle JL, Houghton J, Kearney G (1982) The Cancer Research Campaign (King's/Cambridge) trial for early breast cancer: clinicopathological aspects. Br J Cancer 45:655–609
Feizi T (1985) Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigens. Nature 314:54–57
Hakomori S (1985) Aberrant glycosylation in cancer cell membranes as focused on glycolipids: overview and perspectives. Cancer Res 45:2405–2414
Hawkins RA, Roberts MM, Forest APM (1980) Oestrogen receptors and breast cancer: current status. Br J Surg 67:153–169
Hsu S-M, Raine L, Fanger H (1981) The use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques; a comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Magnani JL, Nilsson B, Brockhaus M, Zopf D, Steplewski Z, Koprowski H, Ginsburg V (1982) A monoclonal antibody- defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II. J Biol Chem 257:14365–14369
Magnani JL, Steplewski Z, Koprowski H, Ginsburg V (1983) Identification of the gastrointestinal and pancreatic cancer-associated antigen detected by monoclonal antibody 19.9 in the sera of patients as mucin. Cancer Res 43:5489–5492
Maynard PV, Davies CJ, Blamey RW, Elston CW, Johnson J, Griffiths K (1978) Relationship between oestrogen receptor content and histological grade in human primary breast tumours. Br J Cancer 38:745–748
Olding LB, Ahren C, Thurin J, Karlsson KA, Svalander C, Koprowski H (1985) Gastrointestinal carcinoma-associated antigen detected by a monoclonal antibody in dysplasia and adenocarcinoma associated with chronic ulcerative colitis. Int J Cancer 36:131–136
Walker RA (1982) Biological markers in human breast carcinoma. J Pathol 137:109–117
Walker RA (1984) The binding of peroxidase-labelled lectins to human breast epithelium III - altered fucose-binding patterns of breast carcinomas and their significance. J Pathol 144:109–117
Walker RA (1985) The binding of peroxidase-labelled lectins to human breast epithelium IV - The reactivity of breast carcinomas to peanut, soy bean andDolichos biflorus agglutinins. J Pathol 145:269–277
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Walker, R.A., Day, S.J. Expression of the antigen detected by the monoclonal antibody Ca 19.9 in human breast tissues. Vichows Archiv A Pathol Anat 409, 375–383 (1986). https://doi.org/10.1007/BF00708254
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DOI: https://doi.org/10.1007/BF00708254