Virchows Archiv A

, Volume 409, Issue 3, pp 349–363 | Cite as

Localized fibrous tumour of serosal surfaces

Immunohistochemical and ultrastructural evidence for a type of mesothelioma
  • John Doucet
  • Irving Dardick
  • John R. Srigley
  • A. W. Peter van Nostrand
  • M. Adeline Bell
  • Harriette J. Kahn


It is uncertain whether localized lesions of serosal membranes have a kinship to mesotheliomas or are truly fibromatous in nature. Ultrastructural and immunohistochemical investigations were carried out on 12 localized benign and malignant pleural and peritoneal tumours from 10 patients. Electron microscopic findings, including the consistent and non-fibroblastic cellular organization of localized neoplasms, the presence of some form of intercellular junctions in 7 of 10 cases, basal lamina deposition in 3 cases, and polarized microvilli in one case indicated a form of mesothelial differentiation. Using monoclonal and polyclonal antibodies, positive immunostaining of tumour cells for cytokeratin peptides was detected in one case, while antibody to vimentin stained four cases. Light microscopic, ultrastructural and immunohistochemical features of one benign localized serosal tumour, with a unique blend of epithelial and spindle cells, provided further evidence for a histogenic link between localized serosal tumours and diffuse epithelial mesotheliomas. On the basis of the current findings and reports in the literature, it would appear that the majority of localized tumours of serosal membranes are a subset of mesothelioma, while a minority are fibromas.

Key words

Mesothelioma Serous membrane Localized tumour Histogenesis Ultrastructure 


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  1. Alvarez-Fernandez E, Diez-Nau MD (1979) Malignant fibrosarcomatous mesothelioma and benign pleural fibroma (localized fibrous mesothelioma) in tissue culture. A comparison of in vitro pattern of growth in relation to the cell of origin. Cancer 43:1658–1663Google Scholar
  2. Benisch B, Peison B, Sobel HJ, Marquet E (1981) Fibrous mesotheliomas (pseudofibroma of the scrotal sac). A light and ultrastructural study. Cancer 47:731–735Google Scholar
  3. Bolen JW, Hammar SP, McNutt MA (1986) Reactive and neoplastic serosal tissue: A light microscopic, ultrastructural, and immunohistochemical study. Am J Surg Pathol 10:34–47Google Scholar
  4. Bolen JW, Thorning D (1980) Mesotheliomas. A light- and electron-microscopical study concerning histogenetic relationships between the epithelial and the mesenchymal variants. Am J Surg Pathol 4:451–464Google Scholar
  5. Briselli M, Mark EJ, Dickersin GR (1981) Solitary fibrous tumors of the pleura: eight new cases and review of the 360 cases in the literature. Cancer 47:2678–2689Google Scholar
  6. Bürrig KF, Kastendieck H (1984) Ultrastructural observations on the histogenesis of localized fibrous tumours of the pleura (benign mesothelioma). Virchows Arch [Pathol Anat] 403:413–424Google Scholar
  7. Dalton WT, Zolliker AS, McCaughey WTE, Jacques J, Kannerstein M (1979) Localized primary tumors of the pleura. An analysis of 40 cases. Cancer 44:1465–1475Google Scholar
  8. Dardick I (1984) Ultrastructure of mesotheliomas. In: Bailey GW (ed) Proceedings of the 42nd Annual Meeting of the Electron Microscopy Society of America, San Francisco Press, San Francisco, pp 98–101Google Scholar
  9. Dardick I, Srigley JR, McCaughey WTE, van Nostrand AWP, Ritchie AC (1984a) Ultrastructural aspects of the histogenesis of diffuse and localized mesothelioma. Virchows Arch [Pathol Anat] 402:373–388Google Scholar
  10. Dardick I, Al-Jabi M, McCaughey WTE, Srigley JR, van Nostrand AWP, Ritchie AC (1984b) Ultrastructure of poorly differentiated diffuse epithelial mesotheliomas. Ultrastruct Pathol 7:151–160Google Scholar
  11. Davis JMG (1974) Histogenesis and fine structure of peritoneal tumors produced in animals by injections of asbestos. J Natl Cancer Inst 52:1823–1837Google Scholar
  12. Hernandez FJ, Fernandez BB (1974) Localized fibrous tumours of pleura: a light and electron microscopic study. Cancer 34:1667–1674Google Scholar
  13. Holden J, Churg A (1984) Immunohistochemical staining for keratin and carcinoembryonic antigen in the diagnosis of malignant mesothelioma. Am J Surg Pathol 8:277–279Google Scholar
  14. Kahn HJ, Huang S-N, Hanna WM, Baumal R, Phillips MJ (1984) Immunohistochemical localization of epidermal and Mallory body cytokeratin in undifferentiated epithelial tumors: Comparison with ultrastructural features. Am J Clin Pathol 81:184–191Google Scholar
  15. Kahn HJ, Hanna W, Yeger H, Baumal R (1982) Immunohistochemical localization of prekeratin filaments in benign and malignant cells in effusions. Comparison with intermediate filament distribution by electron microscopy. Am J Pathol 109:206–214Google Scholar
  16. Kawai T, Suzuki M, Kageyama K (1981) Reactive mesothelial cell and mesothelioma of the pleura. Virchows Arch [Pathol Anat] 393:251–263Google Scholar
  17. Kawai T, Mikita A, Torikata C, Yakumara K, Kageyama K, Shimosato Y (1978) Solitary (localized) pleural mesothelioma. A light and electron microscopic study. Am J Surg Pathol 2:365–375Google Scholar
  18. Kay S, Silverberg SG (1971) Ultrastructural studies of a malignant fibrous mesothelioma of the pleura. Arch Pathol 92:449–455Google Scholar
  19. Kimoff RJ, Huang S-N (1981) Immunocytochemical and immunoelectron microscopic studies on Mallory bodies. Lab Invest 45:491–503Google Scholar
  20. Klemperer P, Rabin CB (1931) Primary neoplasms of the pleura. Arch Pathol 11:385–412Google Scholar
  21. Luse SA, Spjut JH (1964) An electron microscopic study of a solitary pleural mesothelioma. Cancer 17:1546–1554Google Scholar
  22. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R (1982) The catalogue of human cytokeratin polypeptides: Patterns of expression of cytokeratins in normal epithelium, tumours and cultured cells. Cell 31:11–24Google Scholar
  23. Nicosia SV, Johnson JH, Streibel EJ (1985) Growth characteristics of rabbit ovarian mesothelial (surface epithelial) cells. Int J Gynecol Pathol 4:58–74Google Scholar
  24. Osamura RY (1977) Ultrastructure of localized fibrous mesothelioma of the pleura. Cancer 39:139–142Google Scholar
  25. Poroshin KK, Krylou LM (1981) Morphology and histogenesis of the so called fibrous mesothelioma (submesothelial fibroma). Arch Pathol 43:35–39Google Scholar
  26. Raftery AT (1973a) Regeneration of parietal and visceral peritoneum. A light microscopical study. Br J Surg 60:293–299Google Scholar
  27. Raftery AT (1973b) Regeneration of parietal and visceral peritoneum. An electron microscopical study. J Anat 115:375–392Google Scholar
  28. Ryan GB, Grobety J, Majno G (1973) Mesothelial injury and recovery. Am J Pathol 71:93–112Google Scholar
  29. Said JW, Nash G, Banks-Schlegel S, Sassoon AF, Shintaku IP (1984) Localized fibrous mesothelioma: an immunohistochemical and electron microscopic study. Hum Pathol 15:440–443Google Scholar
  30. Scharifker D, Krancko M (1979) Localized fibrous mesothelioma of the pleura (submesothelial fibroma). A clinical study of 18 cases. Cancer 43:627–635Google Scholar
  31. Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabelled antibody enzyme method of immunohistochemistry: preparation and properties of soluable antigen-antibody complex (horseradish peroxidase anti-horseradish peroxidase) and in its use in identification of spirochaetes. J Histochem Cytochem 18:315–333Google Scholar
  32. Sun TT, Green H (1978) Immunofluorescent staining of keratin fibres in cultured cells. Cell 14:369–476Google Scholar
  33. Suzuki Y (1980) Pathol of human malignant mesothelioma. Sem Oncology 8:268–282Google Scholar
  34. Suzuki Y, Churg J, Kannerstein M (1976) Ultrastructure of human malignant diffuse mesothelioma. Am J Pathol 85:241–262Google Scholar
  35. Towbin H, Stachelin T, Gordin J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and applications. Proc Natl Acad Sci 76:4350–4354Google Scholar
  36. Wang N-S (1973) Electron microscopy in the diagnosis of pleural mesotheliomas. Cancer 31:1046–1054Google Scholar
  37. Whitaker D, Papadimitriou J (1985) Mesothelial healing: Morphological and kinetic investigations. J Pathol 145:159–175Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • John Doucet
    • 1
  • Irving Dardick
    • 4
  • John R. Srigley
    • 1
  • A. W. Peter van Nostrand
    • 1
  • M. Adeline Bell
    • 2
  • Harriette J. Kahn
    • 3
  1. 1.Departments of PathologyToronto General HospitalToronto, Ontario
  2. 2.University of OttawaOttawa, Ontario
  3. 3.Women's College Hospital and The Hospital for Sick ChildrenToronto, OntarioCanada
  4. 4.Canadian Tumour Reference CentreOttawa, OntarioCanada

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