Skip to main content
SpringerLink
Log in

Calcium ion uptake induced by cholinergic and α-adrenergic stimulation in isolated cells of rat salivary glands

  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Summary

Adrenaline (10−5 M) and carbamylcholine (10−4 M) stimulate45Ca2+ uptake into isolated cells of rat submandibular gland and parotid glands. In the presence of the α-adrenoreceptor blocking agent phentolamine, adrenaline stimulation of45Ca2+ uptake is abolished. The β-adrenergic stimulant isoproterenol has no effect on45Ca2+ uptake. Carbamylcholine induced45Ca2+ uptake is inhibited by atropine. The Ca2+ ionophore A23187 stimulates45Ca2+ uptake, whereas dibutyryl cyclic adenosine 3′,5′-monophosphate and dibutyryl cyclic guanosine 3′,5′-monophosphate have no effect on45Ca2+ uptake.

A graphical analysis of the45Ca2+ uptake curves reveals at least two phases: a fast phase and a slow phase, both of which are stimulated by adrenaline and carbamylcholine.

The45Ca-exchangeable pool size is increased by adrenaline and carbamylcholine in both the fast and the slow phases.

These results suggest that α-adrenergic and cholinergic agonists act by increasing the rate of Ca2+ transfer into the cells of the parotid and submandibular salivary glands most probably through an increase of the cell membrane permeability for Ca2+.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Amsterdam, A., Jamieson, J. D.: Structural and functional characterization of isolated pancreatic exocrine cells. Proc. Natl. Acad. Sci. U.S.A.69, 3028–3032 (1972)

    Google Scholar 

  2. Babad, H., Ben-Zvi, R., Bdolah, A., Schramm, M.: The mechanism of enzyme secretion by the cell. 4. Effects of inducers, substrates and inhibitors on amylase secretion by rat pariotid slices. Eur. J. Biochem.1, 96–101 (1967)

    Google Scholar 

  3. Batzri, S., Selinger, Z.: Enzyme secretion mediated by the epinephrine β-receptor in rat parotid slices. J. Biol. Chem.248, 356–360 (1973)

    Google Scholar 

  4. Batzri, S., Selinger, Z., Schramm, M., Robinovitch, M. R.: Potassium release mediated by the epinephrine α-receptor in rat parotid slices. J. Biol. Chem.248, 361–368 (1973)

    Google Scholar 

  5. Borle, A. B.: Kinetic analysis of calcium movements in Hela cell cultures. J. Gen. Physiol.53, 43–56 (1969)

    Google Scholar 

  6. Butcher, F. R.: The role of calcium and cyclic nucleotides in α-amylase release from slices of rat parotid: studies with the divalent cation ionophore A23187. Metabolism24, 409–418 (1975)

    Google Scholar 

  7. Dormer, R. L., Ashcroft, S. J. H.: Studies on the role of calcium ions in the stimulation by adrenaline of amylase release from rat parotid. Biochem. I.144, 543–550 (1974)

    Google Scholar 

  8. Douglas, W. W., Poisner, A. M.: The influence of calcium on the secretory response of the submaxillary gland to acetyl-choline or to noradrenaline. J. Physiol. (Lond.)165, 528–541 (1963)

    Google Scholar 

  9. Kondo, S., Schulz, I.: Calcium ion uptake in isolated pancreas cells induced by secretagogues. Biochim. Biophys. Acta419, 76–92 (1976)

    Google Scholar 

  10. Kondo, S., Schulz, I.: Ca2+ fluxes in isolated cells of rat pancreas. Effect of secretagogues and different Ca2+ concentrations. J. Membr. Biol.29, 185–203 (1976)

    Google Scholar 

  11. Leslie, B. A., Putney, J. W. Jr., Sherman, J. M.: α-Adrenergic, β-adrenergic and cholinergic mechanisms for amylase secretion by rat parotid gland in vitro. J. Physiol. (Lond.)260, 351–370 (1976)

    Google Scholar 

  12. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. L.: Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 265–275 (1951)

    Google Scholar 

  13. Nielsen, S. P., Petersen, O. H.: Transport of calcium in the perfused submandibular gland of the cat. J. Physiol. (Lond.)223, 685–697 (1972)

    Google Scholar 

  14. petersen, O. H., Pedersen, G. L.: Membrane effects mediated by alpha- and beta-adrenoceptors in mouse parotid acinar cells. J. Membr. Biol.16, 352–362 (1974)

    Google Scholar 

  15. Petersen, O. H.: Stimulus-secretion coupling of salivary acinar cells: the role of membrane permeability change, Ca2+ and cyclic AMP. In: Stimulus-Secretion Coupling in the Gastrointestinal Tract (R. M. Case and H. Goebell, eds.), pp. 281–291 Lancaster: MTP Press Ltd. 1976

    Google Scholar 

  16. Schramm, M., Naim, E.: Adenyl cyclase of rat parotid gland. J. Biol. Chem.245, 3225–3231 (1970)

    Google Scholar 

  17. Schramm, M., Selinger, Z.: The functions of cyclic AMP and calcium as alternative second messengers in parotid gland and pancreas. J. Cycl. Nucl Res.1, 181–192 (1975)

    Google Scholar 

  18. Schulz, I.: the role of extracellular Ca2+ and cyclic nucleotides in the mechanism of enzyme secretion from the cat pancreas. Pflügers Arch.360, 165–181 (1975)

    Google Scholar 

  19. Selinger, Z., Batzri, S., Eimerl, S., Schramm, M.: Calcium and energy requirements for K+ release mediated by the epinephrine α-receptor in rat parotid slices. J. Biol. Chem.248, 369–372 (1973)

    Google Scholar 

  20. Selinger, Z., Eimerl, S., Schramm, M.: A calcium ionophore simulating the action of epinephrine on the α-adrenergic receptor. Proc. Natl. Acad. Sci. U.S.A.71, 128–131 (1974)

    Google Scholar 

  21. Street, H. V., Close, J. R.: Determination of amylase activity in biological fluids. Clin. Chim. Acta1, 256–268 (1956)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max-Planck-Institut für Biophysik, Kennedyallee 70, D-6000, Frankfurt am Main, Federal Republic of Germany

    H. R. Koelz, S. Kondo, A. L. Blum & I. Schulz

  2. Medizinische Klinik, Stadtspital Triemli, Birmensdorfer-Strasse 497, CH-8063, Zürich, Switzerland

    H. R. Koelz & A. L. Blum

  3. School of Medicine, Dept. of Dermatology, Yamagata University, Yamagata City, Japan

    S. Kondo

Authors
  1. H. R. Koelz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Kondo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. L. Blum
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. Schulz
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by Swiss National Science Foundation Grant No. 3.298.074

Rights and permissions

Reprints and permissions

About this article

Cite this article

Koelz, H.R., Kondo, S., Blum, A.L. et al. Calcium ion uptake induced by cholinergic and α-adrenergic stimulation in isolated cells of rat salivary glands. Pflugers Arch. 370, 37–44 (1977). https://doi.org/10.1007/BF00707943

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00707943

Key words

Search

Navigation