New chelating agents for colorimetric determination of calcium

  • Eddy Chapoteau
  • Bronislaw P. Czech
  • Wolodymyr Zazulak


Two new calcium chromoionophores2 and3 are described and their potential application to colorimetric determination of calcium is assessed. Chromogenic compound2 bears two acetic acid chelating groups and a 4-(4′-nitrophenyl)azophenol chromophore. Its complexation of Ca2+ at pH 9.0 is accompanied by a 109-nm bathochromic shift and increase in the absorptivity. Triaryl chromoionophore3 bears two phosphonic acid chelating groups and the same azophenol chromophore. Compound3 exhibits a 107-nm bathochromic shift of the absorption maximum, an increase in the absorptivity upon complexation with Ca2+, and good selectivity for Ca2+ over Mg2+ at pH 7.0. Both chromoionophores exhibit linear responses from 0 to 4×10−3 M Ca2+.

Key words

Calcium complexation chromogenic chelating agent chromoionophore 


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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Eddy Chapoteau
    • 1
  • Bronislaw P. Czech
    • 1
  • Wolodymyr Zazulak
    • 1
  1. 1.Diagnostics DivisionBayer Corp.TarrytownUSA

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