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Plant recovery from seedling-derived shoot tips ofFaidherbia albida grown in vitro

Abstract

In vitro cultures were initiated from shoots taken from seedlings ofFaidherbia albida on Murashige and Skoog based medium supplemented with different combinations of 6-benzylaminopurine (BA) and α-naphthaleneacetic acid (NAA). The shoots grew and rooted on all media. Rooting and vigorous growth were most successful on medium supplemented with 10−7M NAA alone on which 87% of the shoots formed roots. Seventy-one percent of plantlets which were transferred to soil were successfully established and nodulated with the nativeRhizobium. The procedures provide a basis for the development of in vitro techniques for rapid multiplication and physiological studies of the species.

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References

  • Baumer M (1990) Agroforestry and Desertification. Technical Centre for Agricultural and Rural Cooperation, Wageningen, The Netherlands, 250 pp

    Google Scholar 

  • Charreau C and Vidal P (1965) Influence de l'Acacia albida Del. sur le sol, nutrition minérale et rendements des milsPennisetum an Sénégal. L'Agronomique Tropicale, pp 600–626

  • CTFT (1989)Faidherbia albida (Del.) A. Chev. (Synonym:Acacia Albida Del. A monograph). CTFT, Wageningen, The Netherlands, 66 pp

    Google Scholar 

  • Duhoux E and Davies D (1985) Caulogénèse à partir des Bougeons cotylédonaires d'Acacia albida et influence du saccharose sur la Rhizogénèse. Journal of Plant Physiology 121: 175–180

    Google Scholar 

  • Gholz, HL (1987) Agroforestry: Realities, Possibilities and Potentials. Martinus Nijhoff Publishers/International Centre for Research in Agroforestry (ICRAF), Nairobi, Kenya, 227 pp

    Google Scholar 

  • Kartha KK (1986) Production and indexing of disease-free plants. In: Withers LA and Alderson PG, eds, Plant Tissue Culture and its Agricultural Applications, pp 219–238. Butterworths, London

    Google Scholar 

  • von Maydell, HJ (1983) Trees and Shrubs of the Sahel. Their Characteristics and Uses. Eschborn, German Techn. Coop. Service (GTZ), 525 pp

  • Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497

    Google Scholar 

  • Porteres R (1957) Un arbre vivant à contre saison en Afrique soudano — zambézienne:Faidherbia albida. Science et Nature 19: 24

    Google Scholar 

  • Radwanski SA and Wickens GE (1967) The ecology ofAcacia albida on mantle soils in Zalingei, Jebel Marra, Sudan. Journal of Applied Ecology 4: 569–579

    Google Scholar 

  • Ruredzo TJ (1991) A minimum facility method for in vitro collection ofDigitaria eriantha ssp.pentzii andCynodon dactylon. Tropical Grasslands 25: 56–63

    Google Scholar 

  • Ruredzo TJ and Hanson J (1990) Practical applications of in vitro techniques to forage germplasm. In: Dzowela BH, Said AN, Asrat Wendem-Agenehu and Kategile JA, eds, Utilization of Research Results on Forage and Agricultural By-product Materials as Animal Feed Resources in Africa, pp 578–591. Proceedings of a joint ARNAB/PANESA workshop, International Livestock Centre for Africa (ILCA), Addis Ababa, Ethiopia

  • SAS (1985) SAS/STAT Guide for Personal Computers, Version 6. SAS Institute, Cary, NC, USA

    Google Scholar 

  • Withers LA (1987) In vitro methods for collecting germplasm in the field. FAO/IBPGR Plant Genetic Resources Newsletter 69: 2–6

    Google Scholar 

  • Withers LA (1980) Tissue Culture Storage For Genetic Conservation. IBPGR Technical Report. International Board for Plant Genetic Resources (IBPGR), Rome, Italy, 91 pp

    Google Scholar 

  • Young, A (1989) Agroforestry for Soil Conservation. Science and Practice of Agroforestry, CAB International/International Centre for Research in Agroforestry (ICRAF), Nairobi, Kenya, 276 pp

    Google Scholar 

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Ruredzo, T.J., Hanson, J. Plant recovery from seedling-derived shoot tips ofFaidherbia albida grown in vitro. Agroforest Syst 22, 59–65 (1993). https://doi.org/10.1007/BF00707471

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  • DOI: https://doi.org/10.1007/BF00707471

Key words

  • Faidherbia albida
  • Acacia albida
  • tissue culture
  • in vitro culture
  • biotechnology