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Electrophoretic analyses of proteins, peroxidases, and phosphatases of single and mated cultures of Arthroderma benhamiae

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Zusammenfassung

Extrakte 26 Tage alter Kulturen der (+)- und (−),Stämme vonArthroderma benhamiae (UAMH 2822, 2823) und ihre befruchtete Kreuzung (2822 x 2823) wurden mittels der Polyacrylamide-Gel Elektrophorese Methode auf ihre Proteine, Phosphatasen und Peroxidasen untersucht. Sowohl die (+)- wie auch die (−)-Stämme zeigten 8 Proteinbande, aber die mit Gymnothecien gekreuzten Kulturen zeigten nur 6 Bande. Wenn jedoch die Proteinprofile dieser zwei entgegengesetzten Paarungstypen und befruchteten Gymnothecien miteinander verglichen wurden, wurden Unterschiede in der Lage und Färbungsintensität einiger Bande beobachtet. Die säurehaltigen und alkalischen Phosphatasemuster der (+)- und (−)-Stämme waren ziemlich ähnlich, aber unterschieden sich von den Mustern gekreuzter, befruchteter Gymnothecienkulturen. Peroxidase Isozyme wurden in keinen der beiden Paarungstypen oder in den gekreuzten Gymnothecienkulturen entdeckt.

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Authors and Affiliations

  1. Provincial Laboratory of Public Health and University of Alberta Mold Herbarium and Culture Collection, T6G 2J2, Edmonton, Alberta, Canada

    Awatar S. Sekhon & J. W. Carmichael

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  1. Awatar S. Sekhon
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  2. J. W. Carmichael
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Sekhon, A.S., Carmichael, J.W. Electrophoretic analyses of proteins, peroxidases, and phosphatases of single and mated cultures of Arthroderma benhamiae. Mycopathologia 58, 97–100 (1976). https://doi.org/10.1007/BF00707179

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