Abstract
Red blood cell lysis photosensitized by two non-steroidal anti-inflammatory drugs Naproxen (NAP) and Ketoprofen (KPF) was investigated in the presence of β-cyclodextrin (β-Cyd). The photohaemolysis was inhibited by the addition of β-Cyd both for NAP and, to a lesser extent, for KPF. The protective action was found only in a restricted range of concentration of β-Cyd. Higher amounts of β-Cyd interfered with the resistance of the cell to the osmotic shock induced by the photosensitization process. The complexing action of β-Cyd was ascertained through UV-vis absorption spectroscopy, induced circular dichroism and emission spectroscopy.
The isolated complexes Naproxen-β-Cyd (NAP-β-Cyd) and Ketoprofen-β-Cyd (KPF-β-Cyd) were found to protect from the photosensitized membrane damage induced by the two drugs, even if it occurred only in a limited range of concentration. This suggests a valid tool in alleviating thein vitro phototoxic consequences caused by these compounds, even if care has to be taken in therapeutic administration due to the presence of the uncomplexed β-Cyd.
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De Guidi, G., Condorelli, G., Giuffrida, S. et al. Effect ofβ-cyclodextrin complexation on the photohaemolitic activity induced by Ketoprofen and Naproxen sensitization. Journal of Inclusion Phenomena and Molecular Recognition in Chemistry 15, 43–58 (1993). https://doi.org/10.1007/BF00706473
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DOI: https://doi.org/10.1007/BF00706473