Molecular and General Genetics MGG

, Volume 247, Issue 2, pp 164–168 | Cite as

The molecular analysis ofbrown eye color mutations isolated from geographically discrete populations ofDrosophila melanogaster

  • Eiji Nitasaka
  • Tsuneyuki Yamazaki
  • M. M. Green
Original Paper


A large proportion of spontaneous mutations inDrosophila melanogaster strains of laboratory origin are associated with insertions of mobile DNA elements. As a first step toward determining whether spontaneous laboratory mutations are predictive for mutational events occurring in the wild, recessivebrown (bw) eye color mutants were isolated. By inbreeding the progeny of wild-caughtDrosophila melanogaster females,bw mutations were isolated from seven separate geographic sites distributed among Japan, California, Siberia and Hungary. Among a total of 14 mutations studied, no case of transposon mutagenesis was found. At least 4 mutations are associated with small deletions in thebw gene. The remainder are inseparable from wild-typebw by Southern analysis and are presumed to be basepair changes or very small indels. Although only two spontaneousbw mutants of laboratory origin have been analyzed molecularly, one is a mobile element insertion.

Key words

Spontaneous mutations Natural populations Drosophila melanogaster 


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Eiji Nitasaka
    • 1
  • Tsuneyuki Yamazaki
    • 1
  • M. M. Green
    • 2
  1. 1.Department of Biology, Faculty of ScienceKyushu UniversityFukuokaJapan
  2. 2.Section of Molecular and Cellular BiologyUniversity of CaliforniaDavisUSA

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