Abstract
The production of gluconic acid was carried out with high catalase containing Aspergillus niger mutant. This osmofil strain enables to convert the concentrated solutions of D-glucose (300g/l) to D-gluconic acid without gasing using hydrogen peroxide as oxygen source. A controlled addition of hydrogen peroxide based on the pO2 measurement was performed. The conversion of 300g/l glucose solution was achieved with 7 hours and triple conversion (with biomass recycling) within 27 hours with yield with regard to the substrate over 98%. Kinetics of inactivation of glucose oxidasecatalase complex as a whole was examined. Some general factors influencing the inactivation of glucose oxidase and catalase in mycelium are discussed.
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Rosenberg, M., Švitel, J., Šturdík, E. et al. Gluconic acid production by Aspergillus niger with oxygen supply by hydrogen peroxide. Bioprocess Engineering 7, 309–313 (1992). https://doi.org/10.1007/BF00705160
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DOI: https://doi.org/10.1007/BF00705160