Bioprocess Engineering

, Volume 7, Issue 7, pp 287–290 | Cite as

On using H2 as parameter in studies of mixing

  • E. G. Hörnsten


The effects of oxygen limitation and strict anaeroby on cell proliferation are studied in order to explain earlier observations using H2 as a parameter in surveillance of partial anaeroby in large scale Escherichia coli fermentations. Furthermore, metabolic conditions affecting molecular hydrogen production in general are discussed.


Oxygen Hydrogen Escherichia Coli Waste Water Fermentation 
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  1. 1.
    Cleland, N.; Hörnsten, E. G.; Elwing, N.; Enfors, S.-O.; Lundström, J.: Measurement of hydrogen evolution by oxygen-limited E. coli by means of a hydrogen sensitive Pd-MOS sensor. Appl. Microbiol. Biotechnol. 20 (1984) 268–270Google Scholar
  2. 2.
    Cleland, N.; Enfors, S.-O.: A biological system for studies on mixing in bioreactors. Bioproc. Eng. 2 (1984) 115–120Google Scholar
  3. 3.
    Hörnsten, E. G.; Lundström, I.; Elwing, H.: Some biometrical applications of molecular hydrogen and ammonia determinations by the use of metal-oxide-semiconductor devices. In, Bioinstrumentation; research developments and applications. Ed. D. L. Wise, Butterworths, Boston, pp 47–91Google Scholar
  4. 4.
    Hörnsten, E. G.; Danielsson, B.; Elwing, H.; Lundström, J.: Sensorized on line determinations of molecular hydrogen in Escherichia coli fermentations. Appl. Microbiol. Biotechnol. 24 (1986) 117–121Google Scholar
  5. 5.
    Hörnsten, E. G.; unpublished dataGoogle Scholar
  6. 6.
    Gray, P. P.; Dunnill, P.; Lilly, M. D.: The effect of controlled feeding of glycerol onβ-galactosidase production by Escherichia coli in batch culture. Biotechnol. Bioeng. 15 (1973) 1179–1188Google Scholar
  7. 7.
    Hörnsten, E. G.; Lundström, I.; Nordberg, A.; Mathisen, B.: The use of palladium metal oxide semiconductor structures in quantitative studies of H2 and H2S in processes related to biogas production. Bioproc. Eng. 6 (1991) (in press)Google Scholar
  8. 8.
    Chen, J.; Tannahill, A. L.; Shuler, M. L.: Design of a system for the control of low dissolved oxygen concentrations: Critical oxygen concentrations for Actobacter vinelandii and Escherichia coli. Biotechnol. Bioeng. 27 (1985) 151–155Google Scholar
  9. 9.
    Cleland, N.; Larrson, G.; Enfors, S.-O.: Characterization of a biological test system in studies on insufficient mixing in bioreactors: H2 evolution from E. coli. Bioproc. Eng. 5 (1990) 1–6Google Scholar
  10. 10.
    Tapor, C. W.; Tabor, H.: Polyamines. Ann. Rev. Biochem. 42 (1984) 749–790Google Scholar
  11. 11.
    Tkachenko, A. G.; Chudinov, A. A.; Churilova, N. S.: The role of the intracellular polyamine pool in the regulation of constructive metabolism ofEscherichia coli in the course of aerobic-anaerobic transitions. Mikrobiologiya 58 (1989) 709–715Google Scholar
  12. 12.
    Sawers, R. G.; Ballantines, S. P.; Boxer, D. H.: Differential expression of hydrogenase isoenzymes in Escherichia coli K12. Evidence for a third isoenzyme. J. Bacteriol. 164 (1985) 1324–1331Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • E. G. Hörnsten
    • 1
  1. 1.Laboratory of Applied Physics, Bioprocess Engineering GroupLinköping Institute of TechnologyLinköpingSweden

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