Abstract
Ferulic andp-coumaric acid can be separated from their corresponding aliphatic methyl esters by capillary zone electrophoresis, which allows the convenient determination of feruloyl andp-coumaroyl esterase activities using synthetic esters as substrates. A feruloyl-containing sugar ester from wheat bran was also efficiently separated and used as substrate for the enzyme assays.Penicillium expansum was shown to produce feruloyl/p-coumaroyl esterase activity when grown on wheat bran in solid-state culture.
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Additional information
The authors are with the Food Microbiology Research Division, Department of Agriculture for Northern Ireland, Newforge Lane, Belfast BT9 5PX, UK; A.M. McKay is also affiliated with the Department of Food Science (Microbiology), The Queen's University of Belfast, Newforge Lane, Belfast BT9 5PX, UK.
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Donaghy, J.A., McKay, A.M. Measurement of feruloyl/p-coumaroyl esterase by capillary zone electrophoresis. World J Microbiol Biotechnol 11, 160–162 (1995). https://doi.org/10.1007/BF00704639
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DOI: https://doi.org/10.1007/BF00704639