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Bone cell grafts in bioreactor: a study of feasibility of bone cell autograft in large defects

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Abstract

Cancellous bone cells were isolated from adult dogs, introduced into cell culture, subcultured and grown on hydroxylapatite granules. Cells immobilized on these granules were used to make bioreactors which were implanted in dog ulna diaphyse to fill osseous defects. The bioreactor implantation constituted a bone cell autograft and showed bone formation in a reactor containing cultured cells but not in the control reactor containing hydroxylapatite granules without cells. These results indicate that hydroxylapatite material can be used in bioartificial organs. The properties of hydroxylapatite used in bone reconstruction are due to the cells and extra-cellular matrix immobilized on its surface.

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Authors and Affiliations

  1. Bioland, 132 Route d'Espagne, 31100, Toulouse, France

    P. Frayssinet, I. Primout & N. Rouquet

  2. Ecole nat. vétérinaire de Toulouse, Sce de patho. chirurgicale, Toulouse, France

    A. Autefage

  3. Service d'histologie, Faculté de médecine de Rangueil, Toulouse, France

    A. Guilhem

  4. Service d'orthopédie, CHU Purpan, Toulouse, France

    P. Bonnevialle

Authors
  1. P. Frayssinet
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  2. I. Primout
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  3. N. Rouquet
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  4. A. Autefage
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  5. A. Guilhem
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  6. P. Bonnevialle
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Frayssinet, P., Primout, I., Rouquet, N. et al. Bone cell grafts in bioreactor: a study of feasibility of bone cell autograft in large defects. J Mater Sci: Mater Med 2, 217–221 (1991). https://doi.org/10.1007/BF00703374

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  • DOI: https://doi.org/10.1007/BF00703374

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