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Electrochemical assay of thrombin immobilized on carbon electrodes using an electrogenic and chromogenic substrate

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Abstract

The enzymatic and electrochemical features of an immobilized thrombin chemically modified electrode, operating as a voltammetric biosensor, are compared with the availability of a substrate permitting both an electrochemical and spectrophotometric assay of thrombin. Such a device enables the detection of products issued from the enzymatic hydrolysis inside the diffusion layer of the stationary electrode.

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Authors and Affiliations

  1. Laboratoire de Chimie Bioanalytique, Université de Tours, Parc de Grandmont, 37000, Tours, France

    H. Randriamahazaka & J. M. Nigretto

Authors
  1. H. Randriamahazaka
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  2. J. M. Nigretto
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Randriamahazaka, H., Nigretto, J.M. Electrochemical assay of thrombin immobilized on carbon electrodes using an electrogenic and chromogenic substrate. J Mater Sci: Mater Med 2, 205–207 (1991). https://doi.org/10.1007/BF00703371

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  • DOI: https://doi.org/10.1007/BF00703371

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