Abstract
Human procoagulant factor VIII (FVIII:C), is a protein that participates in the cascade of blood coagulation. It is absent or defective in haemophiliac A patients. Furthermore, about 5%–10% of severely affected patients who have received FVIII concentrate as treatment, are developing antibodies which neutralize FVIII:C. Some functional polymers with suitable chemical substituents fixed on to their macromolecular chain might be used in extracorporeal circulation to reduce the concentration of these antibodies. For this purpose, insoluble polystyrenes bearing sulphonate and various amino acid sulphamide groups have been synthesized. The affinity constants for the anti VIII:C and the IgG were determined in purified solution,K anti VIII:C=108−109 l mol−1 andK IgG=105 l mol−1. Thein vitro removal of the anti VIII:C from haemophiliac patient plasma with a high level of antibodies, was tested on various polystyrene-derivative resins. This has led to the selection of active polymers, such as polystyrene substituted by glutamic dimethyl ester acid and/or by hydroxyproline.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
R. COBCROFT, G. TAMAGNINI and K. M. DORMANDY,J. Clin. Path. 30 (1977) 763.
J. A. PENNER,Nouv. Rev. Fr. Hématol. 260 (1982) 260.
G. W. SLOCOMBE, A. C. NEWLAND, M. P. COLVIN and B. T. COLVIN,Brit. J. Haematol. 47 (1981) 577.
J. L. MARX,Science 219 (1983) 271.
Y. SULTAN, A. BUSSEL, P. MAISONNEUVE, M. POUPENEY, X. SITTY and P. GAJDOS,Transfusion September–October (1979).
H. MESSAIKEH, N. BELLATAR, D. GULINO, Y. SULTAN and J. JOZEFONVICZ, in “Biological and Biomechanical Performance of Biomaterials”, edited by P. Christel, A. Meunier and A. J. C. Lee (Amsterdam, 1986) p. 321.
N. BELLATAR, D. GULINO, J. JOZEFONVICZ and Y. SULTAN, in “Proceedings of the 2nd International Conference on Polymers in Medicine II”, edited by E. Chielliniet al. (Plenum Press, New York, 1986) p. 127.
J. P. REGNAULT, in “Immunologie générale” (Decaire, Montréal, 1988) p. 127.
J. L. BRASH and T. A. HORBETT, in “Proteins at Interfaces” (American Chemical Society, Washington, DC, 1987).
L. VROMAN and A. L. ADAMS,J. Biomed. Mater. Res. 3 (1969) 43.
L. VROMAN, A. L. ADAMS, G. C. FISCHER and P. C. MUNOZ,Blood 55 (1980) 156.
J. INGERSLEV and S. STENBJERG,Brit. J. Haematol. 62 (1986) 325.
J. TURKOVA, in “Specific sorbents for high performance liquid affinity chromatography and large scale isolation of proteinases. Affinity chromatography and related techniques”, edited by T. C. J. Gribnau, J. Visser and R. J. F. Nivard (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Boisson-Vidal, C., Messaikeh, H. & Jozefonvicz, J. Adsorption of human antibodies to factor VIII:C to insoluble modified polystyrene from plasma. J Mater Sci: Mater Med 2, 193–196 (1991). https://doi.org/10.1007/BF00703368
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00703368