Treatment of phenolic wastes byAureobasidium pullulans adhered to the fibrous supports
- 27 Downloads
Biological treatment of waste water containing a large amount of phenol was carried out by using a phenolassimilating fungus,Aureobasidium pullulans No. 14 adhered (“semi-immobilized”) to fibrous asbestos. The column reactor employed for oxidative degradation of phenol consisted of a cylindrical glass column containing plastic nets.
During 27 days operation, it was observed that: 1) The phenol removal capacity of the reactor gradually increased during the first 10 days, reaching a stable level. 2) The best phenol removal capacity (50 mg phenol removed/h/ liter of reactor volume) was obtained when an artificial waste water containing up to 1,200 μg/ml phenol was applied to the reactor. 3) Much higher concentrations of phenol (e.g. 1,700 μg/ml) brought about a marked decrease in the phenol removal capacity (40–50 mg/h/liter). 4) Satisfactorily stable operation was achieved using the semiimmobilized mycelia ofAureobasidium pullulans, whose active state could be checked by observing the thick, black-colored biomass which is characteristic of the genusAureobasidium and covered the plastic nets inside the glass column reactor.
KeywordsBiomass Waste Water Phenol Asbestos Biological Treatment
Unable to display preview. Download preview PDF.
- Beltrame P, Beltrame PL, Carniti P, Pitea D (1980) Kinetics of phenol degradation by activated sludge in a continuous-stirred reactor. J Water Pollut Control Fed 52:126–133Google Scholar
- Brown RG, Hanci LA, Hsiao M (1973) Structure and chemical composition of yeast chlamydospores ofAureobasidium pullulans. Can J Microbiol 19:163–168Google Scholar
- Catley BJ (1971) Utllization of carbon sources byPullularia pullulans for the elaboration of extracellular polysaccharides. Appl Microbiol 22:641–649Google Scholar
- Dominguez JB, Goni FM, Uruburu F (1978) The transition from yeast-like to chlamydospore cells inPullularia pullulans. J Gen Microbiol 108:111–117Google Scholar
- Fukuoka S, Eto H, Mikami E, Ono H (1967) Microbial purification of some specific industrial wastes (VI) The treatment of industrial phenolic resin waste water. J Ferment Technol 45: 159–167Google Scholar
- Gadd FM (1980) Melanin production and differentiation in batch cultures of the polymorphic fungusAureobasidium pullulans. FEMS Microbiol Lett 9:237–240Google Scholar
- Holladay DW, Hancher CW, Scott CD, Chilcote DD (1978) Biodegradation of phenolic waste liquors in stirred-tank, packed-bed, and fluidized-bed bioreactors. J Water Pollut Control Fed 50:2573–2589Google Scholar
- Itoh M, Takahashi S, Iritani M, Kaneko Y (1980) Degradation of three isomers of cresol and monohydroxybenzoate by Eumycetes. Agrie Biol Chem 44:1037–1042Google Scholar
- Sakaguchi K, Okazaki H, Takeuchi M (1955) A note on the comparison of Koji and submerged cultures. J Agric Chem Soc Japan 29:349–353Google Scholar
- Shimizu T, Uno T, Dan Y, Nei N, Ichikawa K (1973) Continuous treatment of waste water containing phenol byCandida tropicalis. J Ferment Technol 51:809–812Google Scholar
- Takahashi S, Itoh M, Tsubaki K, Kaneko Y (1981) Taxonomical identification of phenol- ando-cresol-assimilating fungusAureobasidium pullulans and its growth characteristics in phenol medium with methanol or formaldehyde. Agric Biol Chem 45: 1809–1815Google Scholar
- Wallenfels K, Keilich G, Bechtler G, Freudenberger D (1965) Untersuchungen an Pullulan. IV. Die Klärung des Strukturproblems mit physikalischen, chemischen und enzymatischen Methoden. Biochem Z 341:433–450Google Scholar
- Wase DAJ, Hough JS (1966) Continuous culture of yeast on phenol. J Gen Microbiol 42:13–23Google Scholar