Ecotoxicology

, Volume 1, Issue 1, pp 45–73 | Cite as

Sequential expression of biomarkers in Bluegill Sunfish exposed to contaminated sediment

  • C. W. Theodorakis
  • S. J. D'surney
  • J. W. Bickham
  • T. B. Lyne
  • B. P. Bradley
  • W. E. Hawkins
  • W. L. Farkas
  • J. F. McCarthy
  • L. R. Shugart
Papers

Abstract

The temporal expression of various biological rsponses was determined in Bluegill SunfishLepomis macrochirus exposed under controlled laboratory conditions to sediment containing high concentrations of polynuclear aromatic hydrocarbons, polychlorinated biphenyls and heavy metals. Liver, gill, blood, kidney, brain, spleen and intestine were removed from Sunfish sampled at 1, 2, 4, 8, 16, and 40 weeks post-exposure. Biomarker data were recorded for specific proteins, enzymatic activities, DNA integrity, and histopathology. Biomarkers in the laboratory exposed fish were similar to those of indigenous Sunfish sampled from the site of origin of the contaminated sediment. Several patterns of development of biomarkers over time were also evident. For example, the responses of certain biomarkers are not time-dependent (i.e., intestine and gill ATPase activities) while that of others, such as brain ATPase activity, liver cytochrome P450 and NADPH content, stress proteins, chromatin proteins and DNA strand breaks, fluctuate over time. Still other biomarkers, such as EROD activity, zinc protoporphyrin content of the blood, and DNA adducts, showed marked increases over time. Such patterns need to be considered when comparing laboratory and field results and deciding which biomarkers to use for biomonitoring programs. Implications for natural selection and population/community level responses are also discussed.

Keywords

biomarkers Bluegill sediment pollution EFPC 

Abbreviations

Ache

acetylcholinesterase

ALAD

δ-aminolevulenic acid dehydratase

BaP

benzo[a]pyrene

BaPDE

benzo[a]pyrene diol-epoxide

DDW

double distilled water

EFPC

East Fork Poplar Creek

EROD

ethoxyresorufin-O-deethylase

PAH

polycyclic aromatic hydrocarbon

PCB

polychlorinated biphenyl

PAS

periodic acid Schiff

ZPP

zinc protoporphyrin

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Copyright information

© Chapman & Hall 1992

Authors and Affiliations

  • C. W. Theodorakis
    • 1
  • S. J. D'surney
    • 2
  • J. W. Bickham
    • 3
    • 4
  • T. B. Lyne
    • 5
  • B. P. Bradley
    • 6
  • W. E. Hawkins
    • 7
  • W. L. Farkas
    • 1
  • J. F. McCarthy
    • 2
  • L. R. Shugart
    • 2
  1. 1.Graduate Program in Environmental ToxicologyUniversity of TennesseeKnoxvilleUSA
  2. 2.Environmental Sciences DivisionOak Ridge National LaboratoryOak RidgeUSA
  3. 3.Department of Wildlife and Fisheries SciencesTexas A & M UniversityCollege StationUSA
  4. 4.LGL Ecological Genetics Inc.BryanUSA
  5. 5.Department of Soil and Crop SciencesTexas A & M UniversityCollege StationUSA
  6. 6.Department of Biological SciencesUniversity of Maryland (Baltimore County Campus)CatonsvilleUSA
  7. 7.Gulf Coast Research LaboratoryOcean SpringsUSA

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