Summary
The ultrastructural localization of glucose 6-phosphatase activity was investigated in the proximal convoluted tubule cells of the rat kidney. The reaction product for the enzyme activity was present in the endoplasmic reticulum and nuclear envelope, as reported for the hepatic enzyme and others, but was absent from the brush border, plasma membrane and other organelles. The metabolic significance of the association of this enzyme with the endoplasmic reticulum and the role of the enzyme in the active reabsorption and transport of glucose in the renal tubules are discussed.
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Chiquoine, A. D.: The distribution of glucose-6-phosphatase in the liver and kidney of the mouse. J. Histochem. Cytochem.1, 429–435 (1953).
Ericsson, J. L. E.: On the fine structural demonstration of glucose-6-phosphatase. J. Histochem. Cytochem.14, 361–362 (1966).
Freedland, R. A., Taylor, A. R.: Studies on glucose-6-phosphatase and glutaminase in rat liver and kidney. Biochim. biophys. Acta (Amst.)92, 567–571 (1964).
Goldfischer, S., Essner, E., Novikoff, A. B.: The localization of phosphatase activities at the level of ultrastructure. J. Histochem. Cytochem.12, 72–95 (1964).
Graham, R. C., Karnovsky, M. J.: The early stages absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem.14, 291–302 (1966).
Hanssen, O. E.: Early post mortem renal changes studied in mice with one kidney exteriorized 2. The functional and the early post mortem morphology of the kidney. Acta path. microbiol. scand.49, 297–320 (1960).
Hugon, J. S., Borgers, M., Maestracci, D.: Glucose 6-phosphatase and thiamine pyrophosphatase activities in the jejunal epithelium of the mouse. J. Histochem. Cytochem.18, 361–364 (1970).
Jacobsen, N. O., Jørgensen, F., Thomsen, A. C.: On the localization of some phosphatases in three different segments of the proximal tubules in the rat kidney. J. Histochem. Cytochem.15, 456–469 (1967).
Kanamura, S.: Difference in resistance to glutaraldehyde or formaldehyde fixation between mouse and rat hepatic glucose 6-phosphatase. Acta histochem. cytochem.3, 160–162 (1970).
— Fine structural demonstration of hepatic glucose 6-phosphatase activity after prefixation of fresh frozen sections in glutaraldehyde. J. Histochem. Cytochem.19, 320–321 (1971a).
— Demonstration of glucose 6-phosphatase activity in hepatocytes following transparenchymal perfusion fixation with glutaraldehyde. J. Histochem. Cytochem.19, 386–387 (1971b).
Krebs, H. A.: Renal gluconeogenesis. In: Advances in enzyme regulation (G. Weber), vol. 1, p. 385–400. Oxford: Pergamon 1963.
Lazarus, S. S., Barden, H.: Specificity and ultrastructural localization of pancreatic B cell glucose-6-phosphatase. Diabetes14, 146–156 (1965).
Maunsbach, A. B.: Observations on the segmentation of the proximal tubule in the rat kidney. J. Ultrastruct. Res.16, 239–258 (1966).
Nordlie, R. C., Soodsma, J. F.: Phosphotransferase activities of kidney glucose 6-phosphatase. J. biol. Chem.241, 1719–1724 (1966).
Orrenius, S., Ericsson, J. L. E.: On the relationship of liver glucose-6-phosphatase to the proliferation of endoplasmic reticulum in phenobarbital induction. J. Cell Biol.31, 243–256 (1966).
Rosen, S. I.: The localization of glucose-6-phosphate hydrolysing enzyme in hepatocytes, red blood cells and leukocytes in the liver of the newborn rat. J. Anat. (Lond.)105, 579–584 (1969).
Sabatini, D. D., Bensch, K., Barrnett, R. J.: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol.17, 19–58 (1963).
Saito, T., Ogawa, K.: Ultracytochemical changes of the glucose-6-phosphatase (D-glucose-6phosphate phosphohydrolase) activity in liver cells of the rat treated with phenobarbital. Okajimas Folia anat. jap.44, 11–27 (1967).
Sawaragi, I., Wynn, R. M.: Ultrastructural localization of metabolic enzymes during the human endometrial cycle. Obstet. and Gynec.34, 50–61 (1969).
Tice, L. W., Barrnett, R. J.: The fine structural localization of glucose-6-phosphatase in rat liver. J. Histochem. Cytochem.10, 754–762 (1962).
Wachstein, M.: Histochemical staining reactions of the normally functioning and abnormal kidney. J. Histochem. Cytochem.3, 246–270 (1955).
—, Meisel, E.: On the histochemical demonstration of glucose-6-phosphatase. J. Histochem. Cytochem.4, 592 (1956).
— — A comparative study of enzymatic staining reactions in the rat kidney with necrobiosis induced by ischemia and nephrotoxic agents (mercuhydrine and dl-serine). J. Histochem. Cytochem.5, 204–220 (1957).
Walker, A. M., Bott, P. A., Oliver, J., MacDowell, M. C.: The collection and analysis of fluid from single nephrons of the mammalian kidney. Amer. J. Physiol.134, 580–595 (1941).
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Kanamura, S. Ultrastructural localization of glucose-6-phosphatase activity in proximal convoluted tubule cells of rat kidney. Histochemie 28, 288–295 (1971). https://doi.org/10.1007/BF00702634
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DOI: https://doi.org/10.1007/BF00702634