Summary
Intestinal lipid absorption and transport were investigated in albino rats. The observations point towards the existence of a continuity between plasma membrane invaginations and elements of the Golgi complex on its “mature” face. They also suggest a segregation of lipid droplets by paired Golgi membranes and plasma membrane invaginations. The following way for lipid transport is deduced: lipid droplets moving inside the smooth endoplasmic reticulum accumulate progressively and are condensed in Golgi cisternae of the “forming” face. Their limiting membrane ruptures and liberated lipid droplets are segregated by paired Golgi membranes of the “mature” face or by plasma membrane invaginations. Subsequently the inner of the two “segregating” membranes disappears while the lipid droplet is moved towards the intercellular space inside a canal communicating with this space. The suggestion is made that the Golgi apparatus is of double origin: one component representing a terminal plication of the endoplasmic reticulum; the second one—a terminal plication of the plasma membrane invagination. This concept explains the ultrastructural and histochemical differences between Golgi membranes of the “forming” and “mature” faces of the complex.
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References
Arstilla, A. U., Trump, B. P.: Studies on cellular autophagocytosis. Amer. J. Path.53, 687 (1968).
Barrnett, R. J., Rostgaard, J.: Absorption of particulate lipid by intestinal microvilli. Ann. N. Y. Acad. Sci.132, 13 (1965).
Cardell, R. R., Jr., Badenhausen, S., Porter, K. R.: Intestinal triglyceride absorption in the rat. J. Cell Biol.34, 123 (1967).
De Duve, C., Wattiaux, R.: Functions of lysosomes. Ann. Rev. Physiol.28, 435 (1966).
Doggenweller, C. F., Heuser, J. E.: Ultrastructure of the prawn nerve sheats. Role of fixative and osmotic pressure in vesiculation of thin cytoplasmic laminae. J. Cell Biol.34, 407 (1967).
Dominas, H., Przelecka, A., Sarzala, M. G., Taracha, M.: Alkaline phosphatase in the Golgi region of the intestinal epithelium of the frog, mouse and monkey fed with different diets. Folia histochem. cytochem.1, 313 (1963).
Drum, R. W.: Electron microscopy of paired Golgi structures in the Diatom Pinnularia nobilis. J. Ultrastruct. Res.15, 100 (1966).
Emmel, V. M.: Alkaline phosphatase in the Golgi zone of absorbing cells of the small intestine. Anat. Rec.91, 39 (1945).
Ericsson, J. L. E.: Mechanism of cellular autophagy. In: Lysosomes in biology and pathology (eds. J. T. Dingle, and H. B. Fell), vol. 2, p. 345. Amsterdam: North-Holland Publ. Co. 1969.
Essner, E., Novikoff, A. B.: Cytological studies on two functional hepatomas: interrelations of endoplasmic reticulum, Golgi apparatus and lysosomes. J. Cell Biol.15, 289 (1962).
Franzini-Armstrong, C., Porter, K. R.: Sarcolemmal invaginations constituting the T system in fish muscle fibers. J. Cell Biol.22, 675 (1964).
Frazer, A. C.: Absorption of triglyceride fat from the intestine. Physiol. Rev.26, 103 (1946).
Grove, S. N., Bracker, C. E., Morré, D. J.: Cytomembrane differentiation in the endoplasmic reticulum—Golgi apparatus—vesicle complex. Science161, 171 (1968).
Holt, S. J., Hicks, R. M.: Specific staining methods for enzyme localization at the subcellular level. Brit. med. Bull.18, 214 (1962).
Holtzman, E., Dominitz, R.: Cytochemical studies of lysosomes, Golgi apparatus and endoplasmic reticulum in secretion and protein uptake by adrenal medulla cells of the rat. J. Histochem. Cytochem.16, 320 (1968).
Hugon, J. S., Borgers, M.: Alkaline and acid phosphatase activities in the duodenum of foetal and newborn mice. Summary Reports of the third Internat. Congr. of Histochemistry and Cytochemistry, p. 18. Berlin-Heidelberg-New York: Springer 1968.
Isselbacher, K. J.: Metabolism and transport of lipid by intestinal mucosa. Fed. Proc.24, 16 (1965).
— Biochemical aspects of fat absorption. Gastroenterology50, 78 (1966).
Jersild, R. A., Jr.: A time sequence of fat absorption in the rat jejunum. Amer. J. Anat.118, 135 (1966).
Kuff, E. L., Dalton, A. J.: Biochemical studies of isolated Golgi membranes. In: Subcellular particles (ed. T. Hayashi), p. 114. New York: Ronald Press 1959.
Lacy, D., Taylor, A. B.: Fat absorption by epithelial cells of the small intestine of the rat. Amer. J. Anat.110, 155 (1962).
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol.9, 409 (1961).
Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. (Abstr.) J. appl. Phys.32, 1637 (1961).
Novikoff, A. B., Biempica, L.: Cytochemical and electron microscopic examination of Morris and Reubner hepatomas after several years of transplantation. Gunn Monogr.1, 65 (1966).
— Essner, E., Quintana, N.: Golgi apparatus and lysosomes. Fed. Proc.23, 1010 (1964).
— Goldfischer, S.: Nucleoside phosphatase activity in the Golgi apparatus and its usefulness for cytological studies. Proc. nat. Acad. Sci. (Wash.)47, 802 (1961).
Oledzka-Slotwinska, H., Creemers, J., Desmet, V.: Cytidine monophosphate as substrate for electron microscopic visualization of alkaline phosphatase activity. Histochemie9, 320 (1967).
— Desmet, V.: Participation of the cell membrane in the formation of autophagic vacuoles. Virchows Arch. Abt. B2, 47 (1968).
Palade, G. E.: Studies on the endoplasmic reticulum. II. Simple disposition in cells in situ. J. biophys. biochem. Cytol.1, 567 (1955).
— The organization of living matter. Proc. nat. Acad. Sci. (Wash.)52, 613 (1964).
Palay, S. L., Karlin, L. J.: An electron microscopic study of the intestinal villus. II. The pathway of fat absorption. J. biophys. biochem. Cytol.5, 373 (1959).
Parsons, D. F.: A simple method for obtaining increased contrast in araldite sections by using postfixation staining of tissues with potassium permanganate. J. biophys. biochem. Cytol.11, 492 (1961).
Przelecka, A., Ejsmont, G., Sarzala, M. G., Taracha, M.: Alkaline phosphatase activity and synthesis of intestinal phospholipids. J. Histochem. Cytochem.10, 596 (1962).
Rambourg, A.: Electron microscopic detection of glycoproteins: cell structures stained with phosphotungstic acid at low pH. (Abstr.). Excerpta Med. Internat. Congr. Ser., No 166, 75 (1968).
Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol.17, 208 (1963).
Rosenbluth, J.: Contrast between osmium-fixed and permanganate-fixed toad spinal ganglia. J. Cell Biol.16, 143 (1963).
Rostgaard, J., Barrnett, R. J.: Fine structural observations of the absorption of lipid particles in the small intestine of the rat. Anat. Rec.152, 325 (1965).
Sjöstrand, F. S.: The fine structure of the columnar epithelium of the mouse intestine with special reference to fat absorption. In: Biochemical problems of lipids (ed. A. C. Frazer), vol. 1, p. 91. New York: American Elsevier Publishing Co., Inc. 1963.
Smith, R. E., Farquhar, M. G.: Lysosome function in the regulation of the secretory process in cells of the anterior pituitary gland. J. Cell Biol.31, 319 (1966).
Strauss, E. W.: Electron microscopic study of intestinal fat absorption in vitro from mixed micelles containing linolenic acid, monoolein and bile salt. J. Lipid Res.7, 307 (1966).
Wachstein, M., Meisel, E.: Histochemistry of hepatic phosphatases at a physiologic pH with special reference to the demonstration of bile canaliculi. Amer. J. clin. Path.27, 13 (1957).
Yamada, K.: Aspects of the fine structure of the intrahepatic bile duct epithelium in normal and cholecystectomized mice. J. Morph.124, 1 (1968).
Yamamoto, T.: On the thickness of the unit membrane. J. Cell Biol.17, 413 (1963).
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Oledzka-Słotwińka, H., Desmet, V.J. Electron microscopic and cytochemical study on the role of Golgi elements and plasma membrane of enterocytes in the intestinal lipid transport. Histochemie 28, 276–287 (1971). https://doi.org/10.1007/BF00702633
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DOI: https://doi.org/10.1007/BF00702633