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Immunogenetics

, Volume 31, Issue 1, pp 45–51 | Cite as

Characterization ofH-2 congenic strains using DNA markers

  • Vladimir Vincek
  • Jadranka Sertić
  • Zofia Zaleska-Rutczynska
  • Felipe Figueroa
  • Jan Klein
Article

Abstract

Congenic mouse strains are widely used in mapping traits to specific loci or short chromosomal regions. The precision of the mapping depends on the information available about the length of the differential segment—the segment introduced from the donor into the background strain. Until recently, very few markers flanking the differential locus were known and consequently the length of the foreign segment could only be determined imprecisely. Now, in an attempt to construct a map of the mouse chromosome 17, we have produced a set of DNA markers distributed along the chromosome. These markers provide a new opportunity to measure the length of the differential segment of the congenic strains and thus increase their usefulness for gene mapping. Here we examined the DNA of 96H-2 congenic strains using 30 DNA markers; of these, the most proximal is located roughly 1.5 centiMorgans (cM) from the centromere and the most distal is about 20 cM telomeric from theH-2 complex (the complex itself being some 20 cM from the centromere). The mapping depends on polymorphism among the input strains and can therefore establish only the minimal length of the differential segment. This point is emphasized by the fact that the average observed length of the differential segment is only about one half of the expected values.

Keywords

Gene Mapping Chromosomal Region Mouse Strain Specific Locus Minimal Length 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Vladimir Vincek
    • 1
  • Jadranka Sertić
    • 1
  • Zofia Zaleska-Rutczynska
    • 1
  • Felipe Figueroa
    • 1
  • Jan Klein
    • 1
    • 2
  1. 1.Abteilung ImmungenetikMax-Planck-Institut für BiologieTübingenFederal Republic of Germany
  2. 2.Department of Microbiology and ImmunologyUniversity of Miami School of MedicineMiamiUSA

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