Glycoconjugate Journal

, Volume 13, Issue 5, pp 709–716 | Cite as

Molecular cloning and characterization of the gene encoding rat submandibular gland apomucin,Mucsmg

  • Earl F. Albone
  • Fred K. Hagen
  • Claude Szpirer
  • Lawrence A. Tabak
Papers

Abstract

Mucin glycoproteins are a major constituent of salivary secretions and play a primary role in the protection of the oral cavity. Rat submandibular glands (RSMG) synthesize and secrete a low molecular weight (114 kDa) mucin glycoprotein. We have isolated, partially sequenced, and characterized the gene which encodes the RSMG apomucin. The gene is encoded by three exons of 106 nt, 69 nt, and 991 nt, separated by introns of 921 nt and 12.5 kb. CAAT and TATA elements are present, at −68 and −26, respectively, in the 5′ flanking sequence of the RSMG apomucin gene. The tandem repeat domain present in exon III consists of ten tandem repeats of 39 nt encoding the consensus sequence PTTDSTTPAPTTK. Sequence comparison and organization of the nucleic acid sequence encoding the tandem repeats of two alleles for this gene suggests that the apomucin gene has undergone recombinational events during its evolution. No significant sequence similarity was found with other mucin genes, or with other known salivary gland-specific genes. The gene was localized to rat chromosome 14 using somatic cell hybrids that segregate rat chromosomes. Since this, to our knowledge, represents the first RSMG mucin gene cloned, we have designated this geneMucsmg.

Keywords

mucins O-glycosylation gene-expression 

Abbreviations

RSMG

rat submandibular gland

RSM

rat salivary mucin

GRP

glutamine-glutamic-acid rich protein

nt

nucleotide

kb

kilobase

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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Earl F. Albone
    • 1
  • Fred K. Hagen
    • 1
  • Claude Szpirer
    • 2
  • Lawrence A. Tabak
    • 1
  1. 1.Departments of Dental Research and Biochemistry, School of Medicine and DentistryUniversity of RochesterRochesterUSA
  2. 2.Laboratoire de GénétiqueUniversité Libre de BruxellesBrusselsBelgium

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