, Volume 4, Issue 1, pp 23–38 | Cite as

Effects of media composition on substrate removal by pure and mixed bacterial cultures

  • C. P. Leslie GradyJr.
  • Leslie Cordone
  • Laura Cusack


Continuous culture experiments with identical experimental designs were run with a mixed microbial community of activated sludge origin and an axenic bacterial culture derived from it. Each culture received 2-chlorophenol (2-CP) at a concentration of 160 mg/L as COD and L-lysine at a concentration of 65 mg/L as COD. A factorial experimental design was employed with dilution rate and media composition as the two controlled variables. Three dilution rates were studied: 0.015, 0.0325, and 0.05 h−1. Media composition was changed by adding four biogenic compounds (butyric acid, thymine, glutamic acid and lactose) in equal COD proportions at total concentrations of 0, 34, 225, and 1462 mg/L as COD. The measured variables were the effluent concentrations of 2-CP as measured by the 4-aminoantipyrene test and lysine as measured by the o-diacetylbenzene procedure. The results suggest that community structure and substrate composition play important roles in the response of a microbial community to mixed substrates. The addition of more biogenic substrates to the axenic culture had a deleterious effect on the removal of both lysine and 2-CP, although the effect was much larger on lysine removal. In contrast, additional substrates had a positive effect on the removal of 2-CP by the mixed community and much less of a negative effect on the removal of lysine. The dilution rate at which the cultures were growing had relatively little impact on the responses to the additional substrates.

Key words

2-chlorophenol continuous culture L-lysine mixed microbial community multicomponent substrate 



chemical oxygen demand




dissolved organic carbon


method detection limit


suspended solids


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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • C. P. Leslie GradyJr.
    • 1
  • Leslie Cordone
    • 1
  • Laura Cusack
    • 1
  1. 1.Environmental Systems Engineering, L. G. Rich Environmental Research LaboratoryClemson UniversityClemsonUSA

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