Virchows Archiv A

, Volume 407, Issue 1, pp 33–41 | Cite as

Distribution of neurofilament protein and neuron-specific enolase in peripheral neuronal tumours

  • Atsushi Sasaki
  • Akira Ogawa
  • Yoichi Nakazato
  • Yoichi Ishida


Peripheral neuronal tumours were studied by the peroxidase-antiperoxidase (PAP) method for the presence of the neurofilament protein (NFP) and neuron-specific enolase (NSE). All cases of ganglioneuromas and ganglioneuroblastomas were positive for NFP and NSE. Both markers were observed only in tumour cells showing differentiation towards ganglion cells. Of the 14 cases of neuroblastoma, 8 were positive for NFP and 12 were positive for NSE. NSE was detected in most neuroblastic tumour cells. However, NFP was found in neuroblasts with signs of differentiation, such as nuclear enlargement, but not in immature, small round cells. NFP was present in cell bodies as well as in cytoplasmic processes of partially differentiated neuroblasts. The majority of pseudorosettes showed no NFP stain. Thus, antibodies against both NFP and NSE are useful in the diagnosis of peripheral neuronal tumours. Moreover, the presence of NFP seemed to be related to the degree of tumour cell differentiation.

Key words

Peripheral neuronal tumours Immunohistochemistry Neurofilament protein Neuron-specific enolase Pseudorosette 


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  1. Beckwith JB, Martin RF (1968) Observations on the histopathology of neuroblastomas. J Pediatr Surg 3:106–110Google Scholar
  2. Carlei F, Polak JM, Ceccamea A, Marangos PJ, Dahl D, Cocchia D, Michetti F, Lezoche E, Speranza V (1984) Neuronal and glial markers in tumours of neuroblastic origin. Virchow Arch [Pathol Anat] 404:313–324Google Scholar
  3. Dhillon AP, Rode J, Leathem A (1982) Neurone specific enolase: an aid to the diagnosis of melanoma and neuroblastoma. Histopathology 6:81–92Google Scholar
  4. Gown AM, Vogel AM (1984) Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues. Am J Pathol 114:109–321Google Scholar
  5. Hughes M, Marsden HB, Palmer MK (1974) Histologic patterns of neuroblastoma related to prognosis and clinical staging. Cancer 34:1706–1711Google Scholar
  6. Ishiguro Y, Kato K, Ito T, Nagaya M (1983) Determination of three enolase isozymes and S-100 protein in various tumors in children. Cancer Res 43:6080–6084Google Scholar
  7. Lehto VP, Stenman S, Miettinen M, Dahl D, Virtanen I (1983) Expression of a neuronal type of intermediate filament as a distinguishing feature between oat cell carcinoma and other lung cancers. Am J Pathol 110:113–118Google Scholar
  8. Mäkinen J (1972) Microscopic patterns as a guide to prognosis of neuroblastoma in childhood. Cancer 29:1637–1646Google Scholar
  9. Misugi K, Misugi N, Newton WA, Jr (1968) Fine structural study of neuroblastoma, ganglioneuroblastoma, and pheochromocytoma. Arch Pathol 86:160–170Google Scholar
  10. Mori H, Kurokawa M (1980) Morphological and biochemical characterisation of neurofilaments isolated from the rat peripheral nerve. Biomed Res 1:24–31Google Scholar
  11. Nakajima T, Kameya T, Tsumuraya M, Shimosato Y, Isobe T, Ishioka N, Okuyama T (1983) Immunohistochemical demonstration of neuron-specific enolase in normal and neoplastic tissues. Biomed Res 4:495–504Google Scholar
  12. Nakayama I, Tsuda N, Muta H, Fujii H, Tsuji K, Matsuo T, Takahara O (1975) Fine structural comparison of Ewing's sarcoma with neuroblastoma. Acta Pathol Jpn 25:251–268Google Scholar
  13. Odelstad L, Pahlman S, Nilsson K, Larsson E, Läckgren G, Johansson KE, Hjertén S, Grote G (1981) Neuron-specific enolase in relation to differentiation in human neuroblastoma. Brain Res 224:69–82Google Scholar
  14. Osborn M, Altmannsberger M, Shaw G, Shauer A, Weber K (1982) Various sympathetic derived human tumors differ in neurofilament expression. Use in diagnosis of neuroblastoma, ganglioneuroblastoma and pheochromocytoma. Virchow Arch [Cell Pathol] 40:141–156Google Scholar
  15. Osborn M, Weber K (1983) Biology of disease. Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology. Lab Invest 48:372–394Google Scholar
  16. Romansky SG, Crocker DW, Shaw KNF (1978) Ultrastructural studies on neuroblastoma. Evaluation of cytodifferentiation and correlation of morphology and biochemical and survival data. Cancer 42:2392–2398Google Scholar
  17. Rorke LB (1983) The cerebellar medulloblastoma and its relationship to primitive neuroectodermal tumors. J Neuropathol Exp Neurol 42:1–15Google Scholar
  18. Shaw G, Weber K (1982) Differential expression of neurofilament triplet proteins in brain development. Nature 298:277–279Google Scholar
  19. Tapia FJ, Polak JM, Barbosa AJA, Bloom SR, Marangos PJ, Dermody C, Pearse AGE (1981) Neuron-specific enolase is produced by neuroendocrine tumors. Lancet 11:808–811Google Scholar
  20. Taxy JB (1980) Electron microscopy in the diagnosis of neuroblastoma. Arch Pathol Lab Med 104:355–360Google Scholar
  21. Tazawa K, Soga J, Ito H (1971) Fine structure of neuroblastoma - a case report. Acta Pathol Jpn 21:257–270Google Scholar
  22. Trojanowski JQ, Lee VM-Y (1983) Monoclonal and polyclonal antibodies against neural antigens. Diagnostic applications for studies of central and peripheral nervous system tumors. Hum Pathol 14:281–285Google Scholar
  23. Vinores SA, Bonnin JM, Rubinstein LJ, Marangos PJ (1984) Immunohistochemical demonstration of neuron-specific enolase in neoplasms of the CNS and other tissues. Arch Pathol Lab Med 108:536–540Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Atsushi Sasaki
    • 1
  • Akira Ogawa
    • 1
  • Yoichi Nakazato
    • 1
  • Yoichi Ishida
    • 1
  1. 1.Department of PathologyGunma University School of MedicineGunmaJapan

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