Summary
A mouse monoclonal antibody (mAB lu-5) was prepared using a lung cancer cell line as an antigen. The selected clone produces an IgG with a gamma-1 heavy chain and a kappa-light-chain. Immunohistochemical testing of mAB lu-5 on 117 normal tissue biopsies and 474 tumours revealed reactivity with an intracytoplasmic, formaldehyderesistant antigen present in most epithelial and mesothelial cells, but absent in mesenchymal cells. The antibody can therefore be used as a first order, pan-epithelial marker. It proved also useful for fast tumour diagnosis on frozen sections.
Similar content being viewed by others
References
Brooks JJ (1982) Immunohistochemistry of soft tissue tumours. Human Pathol 13:969–974
Clark HF, Shepard CC (1963) A dialysis technique for preparing fluorescent antibody. Virology 20:642–644
Debus E, Weber K, Osborn M (1982) Monoclonal cytokeratin antibodies that distinguish simple from stratified squamous epithelia: characterization on human tissues. EMBO J 1:1641–1647
Donner L, de Lanerolle P, Costa J (1983) Immunoreactivity of paraffin-embedded normal tissues and mesenchymal tumours for smooth muscle myosin. Am J Clin Pathol 80:677–681
Gigi O, Geiger B, Eshhar Z, Moll R, Schmid E, Winter S, Schiller DL, Franke WW (1982) Detection of a cytokeratin determinant common to diverse epithelial cells by a broadly cross-reacting monoclonal antibody. The EMBO 1:1429–1437
Guesdon JL, Ternynck T, Avrameas S (1979) The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem 27:1131–1139
Heitz PU, Komminoth P, Kasper M, Obrist E, Oberholzer M, von Overbeck J, Gudat F, Müller KH, Klöppel G, Stähli C, Staehelin TH, Höfler H (1984) Differentialdiagnose von Metastasen neuroendokriner Tumoren. Verh. Dtsch Ges Pathol 68:81–82
Huang SN (1975) Immunohistochemical demonstration of hepatitis B core and surface antigens in paraffin sections. Lab Invest 33:88–95
Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497
Lieber M, Smith B, Szakal A, Nelson-Rees W, Todaro G (1976) A continuous tumour-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int J Cancer 17:62–70
Moll R, Franke WW, Schiller DL (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24
Moll R, Levy R, Czernobilsky B, Hohlweg-Majert P, Dallenbach-Hellweg G, Franke WW (1983) Cytokeratins of normal epithelia and some neoplasms of the female genital tract. Lab Invest 49:599–610
Nathrath WBJ, Heidenkummer P, Björklund V, Björklund B (1985) Distribution of tissue polypeptide antigen (TPA) in normal human tissues: immunohistochemical study on unfixed, methanol-, ethanol-, and formalin-fixed tissues. J Histochem Cytochem 33:99–109
Osborn M, Weber K (1983) Biology of disease. Tumor diagnosis by intermediate filament typing: A novel tool for surgical pathology. Lab Invest 48:372–394
Ramaekers F, Huysmans A, Moesker O, Kant A, Jap P, Herman C, Vooijs P (1983) Monoclonal antibody to keratin filaments, specific for Glandular Epithelia and their tumours. Use in surgical Pathology. Lab Invest 49:353–361
Schauer A, Osborn M, Weber K, Altmannsberger M (1984) Antibodies to different intermediate filaments as histogenetic tumormarkers. Acta Histochem, Suppl Bd XXIX:129–136
Stähli C, Staehelin TH, Miggiano V, Schmidt J, Häring P (1980) High frequencies of antigen- speciific hybridomas: Dependence on immunization parameters and prediction by spleen cell analysis. J Immunol Methods 32:297–304
Stähli C, Staehelin TH, Miggiano V (1983) Spleen cell analysis and optimal immunization for high-frequency production of specific hybridomas. Meth Enzym 92:26–36
Stein BS, Petersen RO, Vangore S, Kendall AR (1982) Immunoperoxidase localization of prostate-specific antigen. Am J Surg Pathol 6:553–557
Sternberger LA, Hardy PH jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody-enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Stocker JW, Forster HK, Miggiano V, Stäihli C, Staiger G, Takacs B, Staehelin TH (1982) Generation of 2 new mouse myeloma cell lines “PAI” and “PAI-O” for hybridoma production. Res Dis May:155–157
Tseng SCG, Jarvinen MJ, Nelson WG, Huang JW, Woodcock-Mitchell J, Sun TT (1982) Correlation of specific keratin with different types of epithelial differentiation: monoclonal antibodies studies. Cell 30:361–372
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
von Overbeck, J., Stähli, C., Gudat, F. et al. Immunohistochemical characterization of an anti-epithelial monoclonal antibody (mAB lu-5). Vichows Archiv A Pathol Anat 407, 1–12 (1985). https://doi.org/10.1007/BF00701324
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00701324