Summary
Nonhistone proteins were isolated from human placental and tonsillar chromatins. Antiserum was prepared against a complex from some nonhistone proteins and DNA (NP-DNA) from placental chromatin. With the help of polyacrylamide gel electrophoresis and immunological methods the tissue specificity of human chromatin nonhistone proteins was established. The described organ immunogenic specificity of the complex of DNA and nonhistone proteins (NP-DNA) from human chromatin is in accordance with data published on similar complexes from different animal organs. Besides, it is shown that shearing of chromatin leads to large chifts in NP-DNA concentrations required for maximum complement fixation in the presence of the prepared antiserum. This may probably be due to a damage of certain chromatin super structures which involve some of the nonhistone proteins and DNA sequences from both the more condensed and less condensed parts of chromatin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barret, T., Gould, H.J.: Tissue and species specificity of nonhistone chromatin proteins. Biochim. biophys. Acta (Amst.)294, 165 (1973)
Barret, T., Maryanka, D., Hamlyn, P.H., Gould, H.J.: Nonhistone proteins control gene expression in reconstituted chromatin Proc. nat. Acad. Sci. (Wash.)71, 5057 (1974)
Blobel, G., Potter, V.R.: Nuclei from rat liver: Isolation method that combines purity with high yield. Science154 1662 (1966)
Burton, K.: A study of the conditions and mechanisms of the diphenylamine reactions for the colorimetric estimation of deoxyribonucleic acid. Biochem. J.62, 315 (1956)
Elgin, S.C.R., Bonner, J.: Partial fractionation and chemical characterization of the major nonhistone chromosomal proteins Biochemistry (Wash.)11, 772 (1972)
Garrard, W. T., Bonner, J.: Changes in chromatin proteins during liver regeneration. J. biol. Chem.249, 5570 (1974)
Kostraba, N.C., Wang, T.Y.: Differential activation of transcription of chromatin by nonhistone fractions. Biochim. biophys. Acts (Amst.)262, 169 (1972)
Kostraba, N.G., Montagna, B.A., Wang, T.Y.: Study of the loosely bound nonhistone chromatin protein: Stimulation of deoxyribonucleic acid templated ribonucleic acid synthesis by a specific deoxyribonucleic acid-binding phospheprotein fraction. J. biol. Chem.250, 1548 (1975)
Laemmli, V.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond)227, 680 (1970)
Levine, L., Van Vunakis, H.: Micro complement fixation. Meth. in Enzymol.11, 928 (1967)
Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall R.J.: Protein measurement with the folin phenol reagent. J. biol. Chem.193, 265, (1951)
McGillvray, A.J., Rickwood, D.: The heterogeneity of mouse-chromatin nonhistone proteins as evidenced by two-dimensional polyacrylamide-gel electrophoresis and ion-exchange chromatography. Europ. J. Biochem.41, 181 (1974)
Spelsberg, T.C., Hnilica, L.S., Ansevin, A.T.: Proteins of chromatin in template restiction. III. The macromolecules in specific restriction of the chromatin DNA. Biochim. biophys. Acta (Amst.)228, 550 (1971)
Tsai, H., Tsai, J.H.H., Yu, P.H.: Effect of yeast proteinase and its inhibitor on the inactivation of tryphophan synthase from Saccharomyces cerevisiae and Neurospora crassa. Europ. J. Biochem.40, 225 (1973)
Wakabayashi, K., Wang, S., Hnilica, L.S.: Immunospecificity of nonhistone proteins in chromatin. Biochemistry (Wash.)13, 1027 (1974)
Author information
Authors and Affiliations
Additional information
Communicated by F. Gros
Rights and permissions
About this article
Cite this article
Dewair, M.A., Matthaei, H. Tissue specificity of nonhistone proteins from human chromatin. Molec. Gen. Genet. 146, 309–312 (1976). https://doi.org/10.1007/BF00701256
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00701256