Summary
The binding of λ genecI product to λ DNA was studied at temperatures from 0°C to 46° C. Binding activity of the products ofcIts mutants was higher at 22° C than at 0° C, 26° C or 30° C. BothcI+ andcIts products lost DNA-binding activity at 46° C, but after subsequent cooling to 22° C, they regained 50–100% of their activity.
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Communicated by G. Bertani
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Mandal, N.C., Lieb, M. Heat-sensitive DNA-binding activity of thecI product of bacteriophage lambda. Molec. Gen. Genet. 146, 299–302 (1976). https://doi.org/10.1007/BF00701254
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DOI: https://doi.org/10.1007/BF00701254